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Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus

In bacteria, multicellular behaviors are regulated by cell–cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility...

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Detalles Bibliográficos
Autores principales: Ducret, Adrien, Fleuchot, Betty, Bergam, Ptissam, Mignot, Tâm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721248/
https://www.ncbi.nlm.nih.gov/pubmed/23898400
http://dx.doi.org/10.7554/eLife.00868
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author Ducret, Adrien
Fleuchot, Betty
Bergam, Ptissam
Mignot, Tâm
author_facet Ducret, Adrien
Fleuchot, Betty
Bergam, Ptissam
Mignot, Tâm
author_sort Ducret, Adrien
collection PubMed
description In bacteria, multicellular behaviors are regulated by cell–cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imaging, we show for the first time that transient contacts between two cells are sufficient to transfer OM materials, proteins and lipids, at high efficiency. Transfer was associated with the formation of dynamic OM tubes, strongly suggesting that transfer results from the local fusion of the OMs of two transferring cells. Last, large amounts of OM materials were released in slime trails deposited by gliding cells. Since cells tend to follow trails laid by other cells, slime-driven OM material exchange may be an important stigmergic regulation of Myxococcus social behaviors. DOI: http://dx.doi.org/10.7554/eLife.00868.001
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spelling pubmed-37212482013-07-29 Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus Ducret, Adrien Fleuchot, Betty Bergam, Ptissam Mignot, Tâm eLife Cell Biology In bacteria, multicellular behaviors are regulated by cell–cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imaging, we show for the first time that transient contacts between two cells are sufficient to transfer OM materials, proteins and lipids, at high efficiency. Transfer was associated with the formation of dynamic OM tubes, strongly suggesting that transfer results from the local fusion of the OMs of two transferring cells. Last, large amounts of OM materials were released in slime trails deposited by gliding cells. Since cells tend to follow trails laid by other cells, slime-driven OM material exchange may be an important stigmergic regulation of Myxococcus social behaviors. DOI: http://dx.doi.org/10.7554/eLife.00868.001 eLife Sciences Publications, Ltd 2013-07-23 /pmc/articles/PMC3721248/ /pubmed/23898400 http://dx.doi.org/10.7554/eLife.00868 Text en Copyright © 2013, Ducret et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Ducret, Adrien
Fleuchot, Betty
Bergam, Ptissam
Mignot, Tâm
Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title_full Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title_fullStr Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title_full_unstemmed Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title_short Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus
title_sort direct live imaging of cell–cell protein transfer by transient outer membrane fusion in myxococcus xanthus
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721248/
https://www.ncbi.nlm.nih.gov/pubmed/23898400
http://dx.doi.org/10.7554/eLife.00868
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