Cargando…

Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms

SUMMARY: The purpose of this study was to examine the in vivo effects of Ekebergia capensis leaf ethanolic extract (EKE) on the blood pressure of anaesthetised normotensive male Wistar rats and conscious weanling Dahl salt-sensitive (DSS) rats, which develop hypertension as they age. To investigate...

Descripción completa

Detalles Bibliográficos
Autores principales: Kamadyaapa, David R, Gondwe, Mavuto M, Moodley, Kogi, Musabayane, Cephas T, Ojewole, John AO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Clinics Cardive Publishing 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721315/
https://www.ncbi.nlm.nih.gov/pubmed/19575078
_version_ 1782278060465192960
author Kamadyaapa, David R
Gondwe, Mavuto M
Moodley, Kogi
Musabayane, Cephas T
Ojewole, John AO
author_facet Kamadyaapa, David R
Gondwe, Mavuto M
Moodley, Kogi
Musabayane, Cephas T
Ojewole, John AO
author_sort Kamadyaapa, David R
collection PubMed
description SUMMARY: The purpose of this study was to examine the in vivo effects of Ekebergia capensis leaf ethanolic extract (EKE) on the blood pressure of anaesthetised normotensive male Wistar rats and conscious weanling Dahl salt-sensitive (DSS) rats, which develop hypertension as they age. To investigate possible mechanism(s) of the extract’s hypotensive effects, the contractile or relaxant responses to EKE in the absence or presence of reference drugs were evaluated in Wistar rat isolated aortic rings precontracted with methoxamine hydrochloride (ME, 10 μM). Acute intravenous administration of EKE elicited hypotensive responses in anaesthetised animals, while sub-chronic treatment with the extract averted the development of high blood pressure in weanling DSS rats. Isometric recordings of methoxamine hydrochloride (ME) pre-contracted, isolated, endothelium-intact and -denuded aortic rings revealed concentration-dependent relaxation responses to EKE (1–160 mg/ml). The potency was significantly less in the endothelium-denuded rings. Inhibitors of endothelium-derived relaxing factor (EDRF), L-NAME, methylene blue and indomethacin significantly reduced EKE-evoked vasorelaxations in endothelium-intact aortic rings. These results indicate that the vasorelaxant effect of EKE was in part mediated via EDRF-dependent or -independent pathways. These observations suggest that the hypotensive effect of EKE was in part mediated via modulation of total peripheral resistance of the vascular smooth muscles.
format Online
Article
Text
id pubmed-3721315
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Clinics Cardive Publishing
record_format MEDLINE/PubMed
spelling pubmed-37213152013-08-07 Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms Kamadyaapa, David R Gondwe, Mavuto M Moodley, Kogi Musabayane, Cephas T Ojewole, John AO Cardiovasc J Afr Cardiovascular Topics SUMMARY: The purpose of this study was to examine the in vivo effects of Ekebergia capensis leaf ethanolic extract (EKE) on the blood pressure of anaesthetised normotensive male Wistar rats and conscious weanling Dahl salt-sensitive (DSS) rats, which develop hypertension as they age. To investigate possible mechanism(s) of the extract’s hypotensive effects, the contractile or relaxant responses to EKE in the absence or presence of reference drugs were evaluated in Wistar rat isolated aortic rings precontracted with methoxamine hydrochloride (ME, 10 μM). Acute intravenous administration of EKE elicited hypotensive responses in anaesthetised animals, while sub-chronic treatment with the extract averted the development of high blood pressure in weanling DSS rats. Isometric recordings of methoxamine hydrochloride (ME) pre-contracted, isolated, endothelium-intact and -denuded aortic rings revealed concentration-dependent relaxation responses to EKE (1–160 mg/ml). The potency was significantly less in the endothelium-denuded rings. Inhibitors of endothelium-derived relaxing factor (EDRF), L-NAME, methylene blue and indomethacin significantly reduced EKE-evoked vasorelaxations in endothelium-intact aortic rings. These results indicate that the vasorelaxant effect of EKE was in part mediated via EDRF-dependent or -independent pathways. These observations suggest that the hypotensive effect of EKE was in part mediated via modulation of total peripheral resistance of the vascular smooth muscles. Clinics Cardive Publishing 2009-05 /pmc/articles/PMC3721315/ /pubmed/19575078 Text en Copyright © 2010 Clinics Cardive Publishing http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cardiovascular Topics
Kamadyaapa, David R
Gondwe, Mavuto M
Moodley, Kogi
Musabayane, Cephas T
Ojewole, John AO
Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title_full Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title_fullStr Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title_full_unstemmed Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title_short Cardiovascular effects of Ekebergia capensis Sparrm (Meliaceae) ethanolic leaf extract in experimental animal paradigms
title_sort cardiovascular effects of ekebergia capensis sparrm (meliaceae) ethanolic leaf extract in experimental animal paradigms
topic Cardiovascular Topics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3721315/
https://www.ncbi.nlm.nih.gov/pubmed/19575078
work_keys_str_mv AT kamadyaapadavidr cardiovasculareffectsofekebergiacapensissparrmmeliaceaeethanolicleafextractinexperimentalanimalparadigms
AT gondwemavutom cardiovasculareffectsofekebergiacapensissparrmmeliaceaeethanolicleafextractinexperimentalanimalparadigms
AT moodleykogi cardiovasculareffectsofekebergiacapensissparrmmeliaceaeethanolicleafextractinexperimentalanimalparadigms
AT musabayanecephast cardiovasculareffectsofekebergiacapensissparrmmeliaceaeethanolicleafextractinexperimentalanimalparadigms
AT ojewolejohnao cardiovasculareffectsofekebergiacapensissparrmmeliaceaeethanolicleafextractinexperimentalanimalparadigms