Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspe...

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Autores principales: Schaumann, Reiner, Knoop, Nicolas, Genzel, Gelimer H, Losensky, Kevin, Rosenkranz, Christiane, Stîngu, Catalina S, Schellenberger, Wolfgang, Rodloff, Arne C, Eschrich, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722536/
https://www.ncbi.nlm.nih.gov/pubmed/23919091
http://dx.doi.org/10.2174/1874285801307010118
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author Schaumann, Reiner
Knoop, Nicolas
Genzel, Gelimer H
Losensky, Kevin
Rosenkranz, Christiane
Stîngu, Catalina S
Schellenberger, Wolfgang
Rodloff, Arne C
Eschrich, Klaus
author_facet Schaumann, Reiner
Knoop, Nicolas
Genzel, Gelimer H
Losensky, Kevin
Rosenkranz, Christiane
Stîngu, Catalina S
Schellenberger, Wolfgang
Rodloff, Arne C
Eschrich, Klaus
author_sort Schaumann, Reiner
collection PubMed
description Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.
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spelling pubmed-37225362013-08-05 Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Schaumann, Reiner Knoop, Nicolas Genzel, Gelimer H Losensky, Kevin Rosenkranz, Christiane Stîngu, Catalina S Schellenberger, Wolfgang Rodloff, Arne C Eschrich, Klaus Open Microbiol J Article Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli. Bentham Open 2013-06-28 /pmc/articles/PMC3722536/ /pubmed/23919091 http://dx.doi.org/10.2174/1874285801307010118 Text en © Schaumann et al.; Licensee Bentham Open. http://creativecommons.org/-licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/-licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Schaumann, Reiner
Knoop, Nicolas
Genzel, Gelimer H
Losensky, Kevin
Rosenkranz, Christiane
Stîngu, Catalina S
Schellenberger, Wolfgang
Rodloff, Arne C
Eschrich, Klaus
Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title_full Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title_fullStr Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title_full_unstemmed Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title_short Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry
title_sort discrimination of enterobacteriaceae and non-fermenting gram negative bacilli by maldi-tof mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722536/
https://www.ncbi.nlm.nih.gov/pubmed/23919091
http://dx.doi.org/10.2174/1874285801307010118
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