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Kinetic analysis of the leucyl/phenylalanyl-tRNA-protein transferase with acceptor peptides possessing different N-terminal penultimate residues()

The introduction of non-natural amino acids at the N-terminus of peptides/proteins using leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase) is a useful technique for protein engineering. To accelerate the chemoenzymatic reaction, here we systematically optimized the N-terminal penultimat...

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Detalles Bibliográficos
Autores principales: Kawaguchi, Jun, Maejima, Kumino, Kuroiwa, Hiroyuki, Taki, Masumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722611/
https://www.ncbi.nlm.nih.gov/pubmed/23905007
http://dx.doi.org/10.1016/j.fob.2013.06.001