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Polymerase chain reaction optimization for amplification of Guanine-Cytosine rich templates using buccal cell DNA

CONTEXT: Amplification of Guanine-Cytosine (GC) -rich sequences becomes important in screening and diagnosis of certain genetic diseases such as diseases arising due to expansion of GC-rich trinucleotide repeat regions. However, GC-rich sequences in the genome are refractory to standard polymerase c...

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Detalles Bibliográficos
Autores principales: Bhagya, C. H. W. M. R. Chandrasekara, Wijesundera Sulochana, W. S., Hemamali, N. Perera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722634/
https://www.ncbi.nlm.nih.gov/pubmed/23901197
http://dx.doi.org/10.4103/0971-6866.112898