Cargando…

Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome

Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a type I transmembrane protein belonging to the TNFR superfamily. After activated by its ligand GITRL, GITR could influence the activity of effector and regulatory T cells, participating in the development of seve...

Descripción completa

Detalles Bibliográficos
Autores principales: Gan, Xiaoxia, Feng, Xiaoke, Gu, Lei, Tan, Wenfeng, Sun, Xiaoxuan, Lv, Chengyin, Zhang, Miaojia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722791/
https://www.ncbi.nlm.nih.gov/pubmed/23935647
http://dx.doi.org/10.1155/2013/340751
_version_ 1782278232669683712
author Gan, Xiaoxia
Feng, Xiaoke
Gu, Lei
Tan, Wenfeng
Sun, Xiaoxuan
Lv, Chengyin
Zhang, Miaojia
author_facet Gan, Xiaoxia
Feng, Xiaoke
Gu, Lei
Tan, Wenfeng
Sun, Xiaoxuan
Lv, Chengyin
Zhang, Miaojia
author_sort Gan, Xiaoxia
collection PubMed
description Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a type I transmembrane protein belonging to the TNFR superfamily. After activated by its ligand GITRL, GITR could influence the activity of effector and regulatory T cells, participating in the development of several autoimmune and inflammatory diseases included rheumatoid arthritis and autoimmune thyroid disease. We previously reported that serum GITRL levels are increased in systemic lupus erythematosus (SLE) patients compared with healthy controls (HC). Here, we tested serum soluble GITR (sGITR) and GITRL levels in 41 primary Sjögren's syndrome (pSS) patients and 29 HC by ELISA and correlated sGITR and GITRL levels with clinical and laboratory variables. GITR and GITRL expression in labial salivary glands was detected by immunohistochemistry. pSS patients had significantly increased serum levels of sGITR and GITRL compared with controls (GITR: 5.66 ± 3.56 ng/mL versus 0.50 ± 0.31 ng/mL; P < 0.0001; GITRL: 6.17 ± 7.10 ng/mL versus 0.36 ± 0.28 ng/mL; P < 0.0001). Serum sGITR and GITRL levels were positively correlated with IgG (GITRL: r = 0.6084, P < 0.0001; sGITR: r = 0.6820, P < 0.0001) and ESR (GITRL: r = 0.8315, P < 0.0001; sGITR: r = 0.7448, P < 0.0001). Moreover, GITR and GITRL are readily detected in the lymphocytic foci and periductal areas of the LSGs. In contrast, the LSGs of HC subjects did not express GITR or GITRL. Our findings indicate the possible involvement of GITR-GITRL pathway in the pathogenesis of pSS. Further studies may facilitate the development of targeting this molecule pathway for the treatment of pSS.
format Online
Article
Text
id pubmed-3722791
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37227912013-08-09 Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome Gan, Xiaoxia Feng, Xiaoke Gu, Lei Tan, Wenfeng Sun, Xiaoxuan Lv, Chengyin Zhang, Miaojia Clin Dev Immunol Research Article Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a type I transmembrane protein belonging to the TNFR superfamily. After activated by its ligand GITRL, GITR could influence the activity of effector and regulatory T cells, participating in the development of several autoimmune and inflammatory diseases included rheumatoid arthritis and autoimmune thyroid disease. We previously reported that serum GITRL levels are increased in systemic lupus erythematosus (SLE) patients compared with healthy controls (HC). Here, we tested serum soluble GITR (sGITR) and GITRL levels in 41 primary Sjögren's syndrome (pSS) patients and 29 HC by ELISA and correlated sGITR and GITRL levels with clinical and laboratory variables. GITR and GITRL expression in labial salivary glands was detected by immunohistochemistry. pSS patients had significantly increased serum levels of sGITR and GITRL compared with controls (GITR: 5.66 ± 3.56 ng/mL versus 0.50 ± 0.31 ng/mL; P < 0.0001; GITRL: 6.17 ± 7.10 ng/mL versus 0.36 ± 0.28 ng/mL; P < 0.0001). Serum sGITR and GITRL levels were positively correlated with IgG (GITRL: r = 0.6084, P < 0.0001; sGITR: r = 0.6820, P < 0.0001) and ESR (GITRL: r = 0.8315, P < 0.0001; sGITR: r = 0.7448, P < 0.0001). Moreover, GITR and GITRL are readily detected in the lymphocytic foci and periductal areas of the LSGs. In contrast, the LSGs of HC subjects did not express GITR or GITRL. Our findings indicate the possible involvement of GITR-GITRL pathway in the pathogenesis of pSS. Further studies may facilitate the development of targeting this molecule pathway for the treatment of pSS. Hindawi Publishing Corporation 2013 2013-07-07 /pmc/articles/PMC3722791/ /pubmed/23935647 http://dx.doi.org/10.1155/2013/340751 Text en Copyright © 2013 Xiaoxia Gan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gan, Xiaoxia
Feng, Xiaoke
Gu, Lei
Tan, Wenfeng
Sun, Xiaoxuan
Lv, Chengyin
Zhang, Miaojia
Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title_full Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title_fullStr Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title_full_unstemmed Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title_short Correlation of Increased Blood Levels of GITR and GITRL with Disease Severity in Patients with Primary Sjögren's Syndrome
title_sort correlation of increased blood levels of gitr and gitrl with disease severity in patients with primary sjögren's syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722791/
https://www.ncbi.nlm.nih.gov/pubmed/23935647
http://dx.doi.org/10.1155/2013/340751
work_keys_str_mv AT ganxiaoxia correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT fengxiaoke correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT gulei correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT tanwenfeng correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT sunxiaoxuan correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT lvchengyin correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome
AT zhangmiaojia correlationofincreasedbloodlevelsofgitrandgitrlwithdiseaseseverityinpatientswithprimarysjogrenssyndrome