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Development and Validation of a Multiplex, Real-Time RT PCR Assay for the Simultaneous Detection of Classical and African Swine Fever Viruses

A single-step, multiplex, real-time polymerase chain reaction (RT-PCR) was developed for the simultaneous and differential laboratory diagnosis of Classical swine fever virus (CSFV) and African swine fever virus (ASFV) alongside an exogenous internal control RNA (IC-RNA). Combining a single extracti...

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Detalles Bibliográficos
Autores principales:	Haines, Felicity J., Hofmann, Martin A., King, Donald P., Drew, Trevor W., Crooke, Helen R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724773/ https://www.ncbi.nlm.nih.gov/pubmed/23923045 http://dx.doi.org/10.1371/journal.pone.0071019

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