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Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil

BACKGROUND: Invasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV...

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Autores principales: de Oliveira, Cristina Mendes, Fregnani, José Humberto Tavares Guerreiro, Carvalho, Jesus Paula, Longatto-Filho, Adhemar, Levi, José Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725167/
https://www.ncbi.nlm.nih.gov/pubmed/23883423
http://dx.doi.org/10.1186/1471-2407-13-357
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author de Oliveira, Cristina Mendes
Fregnani, José Humberto Tavares Guerreiro
Carvalho, Jesus Paula
Longatto-Filho, Adhemar
Levi, José Eduardo
author_facet de Oliveira, Cristina Mendes
Fregnani, José Humberto Tavares Guerreiro
Carvalho, Jesus Paula
Longatto-Filho, Adhemar
Levi, José Eduardo
author_sort de Oliveira, Cristina Mendes
collection PubMed
description BACKGROUND: Invasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV. We aimed to provide updated comprehensive data about the HPV types’ distribution in patients with invasive cervical cancer. METHODS: Fresh tumor tissue samples of histologically confirmed invasive cervical cancer were collected from 175 women attending two cancer reference hospitals from São Paulo State: ICESP and Hospital de Câncer de Barretos. HPV detection and genotyping were performed by the Linear Array HPV Genotyping Test (Roche Molecular Diagnostics, Pleasanton,USA). RESULTS: 170 out of 172 valid samples (99%) were HPV DNA positive. The most frequent types were HPV16 (77.6%), HPV18 (12.3%), HPV31 (8.8%), HPV33 (7.1%) and HPV35 (5.9%). Most infections (75%) were caused by individual HPV types. Women with adenocarcinoma were not younger than those with squamous cell carcinoma, as well, as women infected with HPV33 were older than those infected by other HPV types. Some differences between results obtained in the two hospitals were observed: higher overall prevalence of HPV16, absence of single infection by HPV31 and HPV45 was verified in HC-Barretos in comparison to ICESP patients. CONCLUSIONS: To our knowledge, this is one of the largest studies made with fresh tumor tissues of invasive cervical cancer cases in Brazil. This study depicted a distinct HPV genotype distribution between two centers that may reflect the local epidemiology of HPV transmission among these populations. Due to the impact of these findings on cervical cancer preventive strategies, extension of this investigation to routine screening populations is warranted.
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spelling pubmed-37251672013-07-28 Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil de Oliveira, Cristina Mendes Fregnani, José Humberto Tavares Guerreiro Carvalho, Jesus Paula Longatto-Filho, Adhemar Levi, José Eduardo BMC Cancer Research Article BACKGROUND: Invasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV. We aimed to provide updated comprehensive data about the HPV types’ distribution in patients with invasive cervical cancer. METHODS: Fresh tumor tissue samples of histologically confirmed invasive cervical cancer were collected from 175 women attending two cancer reference hospitals from São Paulo State: ICESP and Hospital de Câncer de Barretos. HPV detection and genotyping were performed by the Linear Array HPV Genotyping Test (Roche Molecular Diagnostics, Pleasanton,USA). RESULTS: 170 out of 172 valid samples (99%) were HPV DNA positive. The most frequent types were HPV16 (77.6%), HPV18 (12.3%), HPV31 (8.8%), HPV33 (7.1%) and HPV35 (5.9%). Most infections (75%) were caused by individual HPV types. Women with adenocarcinoma were not younger than those with squamous cell carcinoma, as well, as women infected with HPV33 were older than those infected by other HPV types. Some differences between results obtained in the two hospitals were observed: higher overall prevalence of HPV16, absence of single infection by HPV31 and HPV45 was verified in HC-Barretos in comparison to ICESP patients. CONCLUSIONS: To our knowledge, this is one of the largest studies made with fresh tumor tissues of invasive cervical cancer cases in Brazil. This study depicted a distinct HPV genotype distribution between two centers that may reflect the local epidemiology of HPV transmission among these populations. Due to the impact of these findings on cervical cancer preventive strategies, extension of this investigation to routine screening populations is warranted. BioMed Central 2013-07-24 /pmc/articles/PMC3725167/ /pubmed/23883423 http://dx.doi.org/10.1186/1471-2407-13-357 Text en Copyright © 2013 de Oliveira et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
de Oliveira, Cristina Mendes
Fregnani, José Humberto Tavares Guerreiro
Carvalho, Jesus Paula
Longatto-Filho, Adhemar
Levi, José Eduardo
Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title_full Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title_fullStr Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title_full_unstemmed Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title_short Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil
title_sort human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from brazil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725167/
https://www.ncbi.nlm.nih.gov/pubmed/23883423
http://dx.doi.org/10.1186/1471-2407-13-357
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