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Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice

Human mesenchymal stem cells (hMSC) can home to tumor sites and promote tumor growth. The effects of hMSC on tumor growth are controversial and involvement of hMSC in tumor immunology has not been adequately addressed. Therefore, we investigated whether injection of hMSC affects tumor appearance, gr...

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Autores principales: Ljujic, Biljana, Milovanovic, Marija, Volarevic, Vladislav, Murray, Bridgid, Bugarski, Diana, Przyborski, Stefan, Arsenijevic, Nebojsa, Lukic, Miodrag L., Stojkovic, Miodrag
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725512/
https://www.ncbi.nlm.nih.gov/pubmed/23892388
http://dx.doi.org/10.1038/srep02298
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author Ljujic, Biljana
Milovanovic, Marija
Volarevic, Vladislav
Murray, Bridgid
Bugarski, Diana
Przyborski, Stefan
Arsenijevic, Nebojsa
Lukic, Miodrag L.
Stojkovic, Miodrag
author_facet Ljujic, Biljana
Milovanovic, Marija
Volarevic, Vladislav
Murray, Bridgid
Bugarski, Diana
Przyborski, Stefan
Arsenijevic, Nebojsa
Lukic, Miodrag L.
Stojkovic, Miodrag
author_sort Ljujic, Biljana
collection PubMed
description Human mesenchymal stem cells (hMSC) can home to tumor sites and promote tumor growth. The effects of hMSC on tumor growth are controversial and involvement of hMSC in tumor immunology has not been adequately addressed. Therefore, we investigated whether injection of hMSC affects tumor appearance, growth and metastasis, and anti-tumor immunity in an experimental animal model of metastatic breast cancer. Injection of hMSC in BALB/c mice bearing mammary carcinoma promoted tumor growth and metastasis, which was accompanied by lower cytotoxic activity of splenocytes, NK cells and CD8(+) T cells in vitro. Tumor-bearing mice that received hMSC had significantly lower percentages of CD3(+)NKp46(+) NKT-like, higher percentages of CD4(+)Foxp3(+) T cells, increased serum levels of Th2 and decreased serum levels of Th1 cytokines, and significantly higher number of CD4(+) cells expressing IL-10. These results demonstrate that immunosuppressive environment created by hMSC promoted breast tumor growth and metastasis in mice.
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spelling pubmed-37255122013-07-29 Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice Ljujic, Biljana Milovanovic, Marija Volarevic, Vladislav Murray, Bridgid Bugarski, Diana Przyborski, Stefan Arsenijevic, Nebojsa Lukic, Miodrag L. Stojkovic, Miodrag Sci Rep Article Human mesenchymal stem cells (hMSC) can home to tumor sites and promote tumor growth. The effects of hMSC on tumor growth are controversial and involvement of hMSC in tumor immunology has not been adequately addressed. Therefore, we investigated whether injection of hMSC affects tumor appearance, growth and metastasis, and anti-tumor immunity in an experimental animal model of metastatic breast cancer. Injection of hMSC in BALB/c mice bearing mammary carcinoma promoted tumor growth and metastasis, which was accompanied by lower cytotoxic activity of splenocytes, NK cells and CD8(+) T cells in vitro. Tumor-bearing mice that received hMSC had significantly lower percentages of CD3(+)NKp46(+) NKT-like, higher percentages of CD4(+)Foxp3(+) T cells, increased serum levels of Th2 and decreased serum levels of Th1 cytokines, and significantly higher number of CD4(+) cells expressing IL-10. These results demonstrate that immunosuppressive environment created by hMSC promoted breast tumor growth and metastasis in mice. Nature Publishing Group 2013-07-29 /pmc/articles/PMC3725512/ /pubmed/23892388 http://dx.doi.org/10.1038/srep02298 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Ljujic, Biljana
Milovanovic, Marija
Volarevic, Vladislav
Murray, Bridgid
Bugarski, Diana
Przyborski, Stefan
Arsenijevic, Nebojsa
Lukic, Miodrag L.
Stojkovic, Miodrag
Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title_full Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title_fullStr Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title_full_unstemmed Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title_short Human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
title_sort human mesenchymal stem cells creating an immunosuppressive environment and promote breast cancer in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725512/
https://www.ncbi.nlm.nih.gov/pubmed/23892388
http://dx.doi.org/10.1038/srep02298
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