Cargando…

Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells

High doses of acetaminophen (APAP; N-acetyl-p-aminophenol) cause severe hepatotoxicity after metabolic activation by cytochrome P450 2E1. This study was undertaken to examine the preventive effects of thiacremonone, a compound extracted from garlic, on APAP-induced acute hepatic failure in male C57B...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Yu Ri, Lee, Nam Jin, Ban, Jung Ok, Yoo, Hwan Soo, Lee, Yong Moon, Yoon, Yeo Pyo, Eum, So Young, Jeong, Heon Sang, Yoon, Do-young, Han, Sang Bae, Hong, Jin Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725907/
https://www.ncbi.nlm.nih.gov/pubmed/23935693
http://dx.doi.org/10.1155/2013/974794
_version_ 1782278598286114816
author Kim, Yu Ri
Lee, Nam Jin
Ban, Jung Ok
Yoo, Hwan Soo
Lee, Yong Moon
Yoon, Yeo Pyo
Eum, So Young
Jeong, Heon Sang
Yoon, Do-young
Han, Sang Bae
Hong, Jin Tae
author_facet Kim, Yu Ri
Lee, Nam Jin
Ban, Jung Ok
Yoo, Hwan Soo
Lee, Yong Moon
Yoon, Yeo Pyo
Eum, So Young
Jeong, Heon Sang
Yoon, Do-young
Han, Sang Bae
Hong, Jin Tae
author_sort Kim, Yu Ri
collection PubMed
description High doses of acetaminophen (APAP; N-acetyl-p-aminophenol) cause severe hepatotoxicity after metabolic activation by cytochrome P450 2E1. This study was undertaken to examine the preventive effects of thiacremonone, a compound extracted from garlic, on APAP-induced acute hepatic failure in male C57BL/6J. Mice received with 500 mg/kg APAP after a 7-day pretreatment with thiacremonone (10–50 mg/kg). Thiacremonone inhibited the APAP-induced serum ALT and AST levels in a dose-dependent manner, and markedly reduced the restricted area of necrosis and inflammation by administration of APAP. Thiacremonone also inhibited the APAP-induced depletion of intracellular GSH, induction of nitric oxide, and lipid peroxidation as well as expression of P450 2E1. After APAP injection, the numbers of Kupffer cells, natural killer cells, and cytotoxic T cells were elevated, but the elevated cell numbers in the liver were reduced in thiacremonone pretreated mice. The expression levels of I-309, M-CSF, MIG, MIP-1α, MIP-1β, IL-7, and IL-17 were increased by APAP treatment, which were inhibited in thiacremonone pretreated mice. These data indicate that thiacremonone could be a useful agent for the treatment of drug-induced hepatic failure and that the reduction of cytotoxic immune cells as well as proinflammatory cytokine production may be critical for the prevention of APAP-induced acute liver toxicity.
format Online
Article
Text
id pubmed-3725907
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37259072013-08-09 Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells Kim, Yu Ri Lee, Nam Jin Ban, Jung Ok Yoo, Hwan Soo Lee, Yong Moon Yoon, Yeo Pyo Eum, So Young Jeong, Heon Sang Yoon, Do-young Han, Sang Bae Hong, Jin Tae Evid Based Complement Alternat Med Research Article High doses of acetaminophen (APAP; N-acetyl-p-aminophenol) cause severe hepatotoxicity after metabolic activation by cytochrome P450 2E1. This study was undertaken to examine the preventive effects of thiacremonone, a compound extracted from garlic, on APAP-induced acute hepatic failure in male C57BL/6J. Mice received with 500 mg/kg APAP after a 7-day pretreatment with thiacremonone (10–50 mg/kg). Thiacremonone inhibited the APAP-induced serum ALT and AST levels in a dose-dependent manner, and markedly reduced the restricted area of necrosis and inflammation by administration of APAP. Thiacremonone also inhibited the APAP-induced depletion of intracellular GSH, induction of nitric oxide, and lipid peroxidation as well as expression of P450 2E1. After APAP injection, the numbers of Kupffer cells, natural killer cells, and cytotoxic T cells were elevated, but the elevated cell numbers in the liver were reduced in thiacremonone pretreated mice. The expression levels of I-309, M-CSF, MIG, MIP-1α, MIP-1β, IL-7, and IL-17 were increased by APAP treatment, which were inhibited in thiacremonone pretreated mice. These data indicate that thiacremonone could be a useful agent for the treatment of drug-induced hepatic failure and that the reduction of cytotoxic immune cells as well as proinflammatory cytokine production may be critical for the prevention of APAP-induced acute liver toxicity. Hindawi Publishing Corporation 2013 2013-07-11 /pmc/articles/PMC3725907/ /pubmed/23935693 http://dx.doi.org/10.1155/2013/974794 Text en Copyright © 2013 Yu Ri Kim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Yu Ri
Lee, Nam Jin
Ban, Jung Ok
Yoo, Hwan Soo
Lee, Yong Moon
Yoon, Yeo Pyo
Eum, So Young
Jeong, Heon Sang
Yoon, Do-young
Han, Sang Bae
Hong, Jin Tae
Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title_full Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title_fullStr Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title_full_unstemmed Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title_short Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
title_sort curative effects of thiacremonone against acetaminophen-induced acute hepatic failure via inhibition of proinflammatory cytokines production and infiltration of cytotoxic immune cells and kupffer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725907/
https://www.ncbi.nlm.nih.gov/pubmed/23935693
http://dx.doi.org/10.1155/2013/974794
work_keys_str_mv AT kimyuri curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT leenamjin curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT banjungok curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT yoohwansoo curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT leeyongmoon curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT yoonyeopyo curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT eumsoyoung curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT jeongheonsang curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT yoondoyoung curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT hansangbae curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells
AT hongjintae curativeeffectsofthiacremononeagainstacetaminopheninducedacutehepaticfailureviainhibitionofproinflammatorycytokinesproductionandinfiltrationofcytotoxicimmunecellsandkupffercells