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Production of the Bacillus licheniformis SubC protease using Lactococcus lactis NICE expression system

In this work the subC gene from Bacillus licheniformis encoding subtilisin was cloned into the nisin-controlled expression (NICE) vectors (pNZ8048 and pNZ8148) with or without the signal peptide SP Usp45 directing extracellular secretion via Sec machinery. Extracellular protease production and activ...

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Detalles Bibliográficos
Autores principales: Mirończuk, Aleksandra M, Krasowska, Anna, Murzyn, Anna, Płachetka, Małgorzata, Łukaszewicz, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing AG 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725919/
https://www.ncbi.nlm.nih.gov/pubmed/23961373
http://dx.doi.org/10.1186/2193-1801-1-54