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Production of the Bacillus licheniformis SubC protease using Lactococcus lactis NICE expression system
In this work the subC gene from Bacillus licheniformis encoding subtilisin was cloned into the nisin-controlled expression (NICE) vectors (pNZ8048 and pNZ8148) with or without the signal peptide SP Usp45 directing extracellular secretion via Sec machinery. Extracellular protease production and activ...
Autores principales: | Mirończuk, Aleksandra M, Krasowska, Anna, Murzyn, Anna, Płachetka, Małgorzata, Łukaszewicz, Marcin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing AG
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3725919/ https://www.ncbi.nlm.nih.gov/pubmed/23961373 http://dx.doi.org/10.1186/2193-1801-1-54 |
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