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Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974

BACKGROUND: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile...

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Autores principales: Wyres, Kelly L, van Tonder, Andries, Lambertsen, Lotte M, Hakenbeck, Regine, Parkhill, Julian, Bentley, Stephen D, Brueggemann, Angela B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726389/
https://www.ncbi.nlm.nih.gov/pubmed/23879707
http://dx.doi.org/10.1186/1471-2164-14-500
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author Wyres, Kelly L
van Tonder, Andries
Lambertsen, Lotte M
Hakenbeck, Regine
Parkhill, Julian
Bentley, Stephen D
Brueggemann, Angela B
author_facet Wyres, Kelly L
van Tonder, Andries
Lambertsen, Lotte M
Hakenbeck, Regine
Parkhill, Julian
Bentley, Stephen D
Brueggemann, Angela B
author_sort Wyres, Kelly L
collection PubMed
description BACKGROUND: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements. RESULTS: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes. CONCLUSIONS: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability.
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spelling pubmed-37263892013-07-30 Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974 Wyres, Kelly L van Tonder, Andries Lambertsen, Lotte M Hakenbeck, Regine Parkhill, Julian Bentley, Stephen D Brueggemann, Angela B BMC Genomics Research Article BACKGROUND: Antimicrobial resistance among pneumococci has greatly increased over the past two to three decades. Resistance to tetracycline (tet(M)), chloramphenicol (cat) and macrolides (erm(B) and/or mef(A/E)) is generally conferred by acquisition of specific genes that are associated with mobile genetic elements, including those of the Tn916 and Tn5252 families. The first tetracycline-, chloramphenicol- and macrolide-resistant pneumococci were detected between 1962 and 1970; however, until now the oldest pneumococcus shown to harbour Tn916 and/or Tn5252 was isolated in 1974. In this study the genomes of 38 pneumococci isolated prior to 1974 were probed for the presence of tet(M), cat, erm(B), mef(A/E) and int (integrase) to indicate the presence of Tn916/Tn5252-like elements. RESULTS: Two Tn916-like, tet(M)-containing, elements were identified among pneumococci dated 1967 and 1968. The former element was highly similar to that of the PMEN1 multidrug-resistant, globally-distributed pneumococcal reference strain, which was isolated in 1984. The latter element was associated with a streptococcal phage. A third, novel genetic element, designated ICESpPN1, was identified in the genome of an isolate dated 1972. ICESpPN1 contained a region of similarity to Tn5252, a region of similarity to a pneumococcal pathogenicity island and novel lantibiotic synthesis/export-associated genes. CONCLUSIONS: These data confirm the existence of pneumococcal Tn916 elements in the first decade within which pneumococcal tetracycline resistance was described. Furthermore, the discovery of ICESpPN1 demonstrates the dynamic variability of pneumococcal genetic elements and is contrasted with the evidence for Tn916 stability. BioMed Central 2013-07-24 /pmc/articles/PMC3726389/ /pubmed/23879707 http://dx.doi.org/10.1186/1471-2164-14-500 Text en Copyright © 2013 Wyres et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wyres, Kelly L
van Tonder, Andries
Lambertsen, Lotte M
Hakenbeck, Regine
Parkhill, Julian
Bentley, Stephen D
Brueggemann, Angela B
Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title_full Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title_fullStr Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title_full_unstemmed Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title_short Evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
title_sort evidence of antimicrobial resistance-conferring genetic elements among pneumococci isolated prior to 1974
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726389/
https://www.ncbi.nlm.nih.gov/pubmed/23879707
http://dx.doi.org/10.1186/1471-2164-14-500
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