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Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cycli...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726613/ https://www.ncbi.nlm.nih.gov/pubmed/23923059 http://dx.doi.org/10.1371/journal.pone.0071267 |
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author | Kalivoda, Eric J. Brothers, Kimberly M. Stella, Nicholas A. Schmitt, Matthew J. Shanks, Robert M. Q. |
author_facet | Kalivoda, Eric J. Brothers, Kimberly M. Stella, Nicholas A. Schmitt, Matthew J. Shanks, Robert M. Q. |
author_sort | Kalivoda, Eric J. |
collection | PubMed |
description | Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cyclic 3′,5′-adenosine monophosphate (cAMP)-cAMP receptor protein (CRP) complex. This study uses S. marcescens as a model system to test the role of cAMP-phosphodiesterase activity in controlling biofilm formation. Herein we describe the characterization of a putative S. marcescens cAMP-phosphodiesterase gene (SMA3506), designated as cpdS, and demonstrated to be a functional cAMP-phosphodiesterase both in vitro and in vivo. Deletion of cpdS resulted in defective biofilm formation and reduced type I fimbriae production, whereas multicopy expression of cpdS conferred a type I fimbriae-dependent hyper-biofilm. Together, these results support a model in which bacterial cAMP-phosphodiesterase activity modulates biofilm formation. |
format | Online Article Text |
id | pubmed-3726613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37266132013-08-06 Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation Kalivoda, Eric J. Brothers, Kimberly M. Stella, Nicholas A. Schmitt, Matthew J. Shanks, Robert M. Q. PLoS One Research Article Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cyclic 3′,5′-adenosine monophosphate (cAMP)-cAMP receptor protein (CRP) complex. This study uses S. marcescens as a model system to test the role of cAMP-phosphodiesterase activity in controlling biofilm formation. Herein we describe the characterization of a putative S. marcescens cAMP-phosphodiesterase gene (SMA3506), designated as cpdS, and demonstrated to be a functional cAMP-phosphodiesterase both in vitro and in vivo. Deletion of cpdS resulted in defective biofilm formation and reduced type I fimbriae production, whereas multicopy expression of cpdS conferred a type I fimbriae-dependent hyper-biofilm. Together, these results support a model in which bacterial cAMP-phosphodiesterase activity modulates biofilm formation. Public Library of Science 2013-07-29 /pmc/articles/PMC3726613/ /pubmed/23923059 http://dx.doi.org/10.1371/journal.pone.0071267 Text en © 2013 Kalivoda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kalivoda, Eric J. Brothers, Kimberly M. Stella, Nicholas A. Schmitt, Matthew J. Shanks, Robert M. Q. Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title | Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title_full | Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title_fullStr | Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title_full_unstemmed | Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title_short | Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation |
title_sort | bacterial cyclic amp-phosphodiesterase activity coordinates biofilm formation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726613/ https://www.ncbi.nlm.nih.gov/pubmed/23923059 http://dx.doi.org/10.1371/journal.pone.0071267 |
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