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Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation

Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cycli...

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Autores principales: Kalivoda, Eric J., Brothers, Kimberly M., Stella, Nicholas A., Schmitt, Matthew J., Shanks, Robert M. Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726613/
https://www.ncbi.nlm.nih.gov/pubmed/23923059
http://dx.doi.org/10.1371/journal.pone.0071267
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author Kalivoda, Eric J.
Brothers, Kimberly M.
Stella, Nicholas A.
Schmitt, Matthew J.
Shanks, Robert M. Q.
author_facet Kalivoda, Eric J.
Brothers, Kimberly M.
Stella, Nicholas A.
Schmitt, Matthew J.
Shanks, Robert M. Q.
author_sort Kalivoda, Eric J.
collection PubMed
description Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cyclic 3′,5′-adenosine monophosphate (cAMP)-cAMP receptor protein (CRP) complex. This study uses S. marcescens as a model system to test the role of cAMP-phosphodiesterase activity in controlling biofilm formation. Herein we describe the characterization of a putative S. marcescens cAMP-phosphodiesterase gene (SMA3506), designated as cpdS, and demonstrated to be a functional cAMP-phosphodiesterase both in vitro and in vivo. Deletion of cpdS resulted in defective biofilm formation and reduced type I fimbriae production, whereas multicopy expression of cpdS conferred a type I fimbriae-dependent hyper-biofilm. Together, these results support a model in which bacterial cAMP-phosphodiesterase activity modulates biofilm formation.
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spelling pubmed-37266132013-08-06 Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation Kalivoda, Eric J. Brothers, Kimberly M. Stella, Nicholas A. Schmitt, Matthew J. Shanks, Robert M. Q. PLoS One Research Article Biofilm-related infections are a major contributor to human disease, and the capacity for surface attachment and biofilm formation are key attributes for the pathogenesis of microbes. Serratia marcescens type I fimbriae-dependent biofilms are coordinated by the adenylate cyclase, CyaA, and the cyclic 3′,5′-adenosine monophosphate (cAMP)-cAMP receptor protein (CRP) complex. This study uses S. marcescens as a model system to test the role of cAMP-phosphodiesterase activity in controlling biofilm formation. Herein we describe the characterization of a putative S. marcescens cAMP-phosphodiesterase gene (SMA3506), designated as cpdS, and demonstrated to be a functional cAMP-phosphodiesterase both in vitro and in vivo. Deletion of cpdS resulted in defective biofilm formation and reduced type I fimbriae production, whereas multicopy expression of cpdS conferred a type I fimbriae-dependent hyper-biofilm. Together, these results support a model in which bacterial cAMP-phosphodiesterase activity modulates biofilm formation. Public Library of Science 2013-07-29 /pmc/articles/PMC3726613/ /pubmed/23923059 http://dx.doi.org/10.1371/journal.pone.0071267 Text en © 2013 Kalivoda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kalivoda, Eric J.
Brothers, Kimberly M.
Stella, Nicholas A.
Schmitt, Matthew J.
Shanks, Robert M. Q.
Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title_full Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title_fullStr Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title_full_unstemmed Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title_short Bacterial Cyclic AMP-Phosphodiesterase Activity Coordinates Biofilm Formation
title_sort bacterial cyclic amp-phosphodiesterase activity coordinates biofilm formation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726613/
https://www.ncbi.nlm.nih.gov/pubmed/23923059
http://dx.doi.org/10.1371/journal.pone.0071267
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