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Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation
BACKGROUND: Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726700/ https://www.ncbi.nlm.nih.gov/pubmed/23922858 http://dx.doi.org/10.1371/journal.pone.0069927 |
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author | Kumar, Dayanidhi Salian, Sujith Raj Kalthur, Guruprasad Uppangala, Shubhashree Kumari, Sandhya Challapalli, Srinivas Chandraguthi, Srinidhi Gururajarao Krishnamurthy, Hanumanthappa Jain, Navya Kumar, Pratap Adiga, Satish Kumar |
author_facet | Kumar, Dayanidhi Salian, Sujith Raj Kalthur, Guruprasad Uppangala, Shubhashree Kumari, Sandhya Challapalli, Srinivas Chandraguthi, Srinidhi Gururajarao Krishnamurthy, Hanumanthappa Jain, Navya Kumar, Pratap Adiga, Satish Kumar |
author_sort | Kumar, Dayanidhi |
collection | PubMed |
description | BACKGROUND: Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear an increased risk of hematological malignancies. OBJECTIVES: To find the influence of occupational radiation exposure on semen characteristics, including genetic and epigenetic integrity of spermatozoa in a chronically exposed population. METHODS: This cross sectional study included 134 male volunteers of which 83 were occupationally exposed to ionizing radiation and 51 were non-exposed control subjects. Semen characteristics, sperm DNA fragmentation, aneuploidy and incidence of global hypermethylation in the spermatozoa were determined and compared between the non-exposed and the exposed group. RESULTS: Direct comparison of the semen characteristics between the non-exposed and the exposed population revealed significant differences in motility characteristics, viability, and morphological abnormalities (P<0.05–0.0001). Although, the level of sperm DNA fragmentation was significantly higher in the exposed group as compared to the non-exposed group (P<0.05–0.0001), the incidence of sperm aneuploidy was not statistically different between the two groups. However, a significant number of hypermethylated spermatozoa were observed in the exposed group in comparison to non-exposed group (P<0.05). CONCLUSIONS: We provide the first evidence on the detrimental effects of occupational radiation exposure on functional, genetic and epigenetic integrity of sperm in health workers. However, further studies are required to confirm the potential detrimental effects of ionizing radiation in these subjects. |
format | Online Article Text |
id | pubmed-3726700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37267002013-08-06 Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation Kumar, Dayanidhi Salian, Sujith Raj Kalthur, Guruprasad Uppangala, Shubhashree Kumari, Sandhya Challapalli, Srinivas Chandraguthi, Srinidhi Gururajarao Krishnamurthy, Hanumanthappa Jain, Navya Kumar, Pratap Adiga, Satish Kumar PLoS One Research Article BACKGROUND: Cytogenetic studies have demonstrated that low levels of chronic radiation exposure can potentially increase the frequency of chromosomal aberrations and aneuploidy in somatic cells. Epidemiological studies have shown that health workers occupationally exposed to ionizing radiation bear an increased risk of hematological malignancies. OBJECTIVES: To find the influence of occupational radiation exposure on semen characteristics, including genetic and epigenetic integrity of spermatozoa in a chronically exposed population. METHODS: This cross sectional study included 134 male volunteers of which 83 were occupationally exposed to ionizing radiation and 51 were non-exposed control subjects. Semen characteristics, sperm DNA fragmentation, aneuploidy and incidence of global hypermethylation in the spermatozoa were determined and compared between the non-exposed and the exposed group. RESULTS: Direct comparison of the semen characteristics between the non-exposed and the exposed population revealed significant differences in motility characteristics, viability, and morphological abnormalities (P<0.05–0.0001). Although, the level of sperm DNA fragmentation was significantly higher in the exposed group as compared to the non-exposed group (P<0.05–0.0001), the incidence of sperm aneuploidy was not statistically different between the two groups. However, a significant number of hypermethylated spermatozoa were observed in the exposed group in comparison to non-exposed group (P<0.05). CONCLUSIONS: We provide the first evidence on the detrimental effects of occupational radiation exposure on functional, genetic and epigenetic integrity of sperm in health workers. However, further studies are required to confirm the potential detrimental effects of ionizing radiation in these subjects. Public Library of Science 2013-07-29 /pmc/articles/PMC3726700/ /pubmed/23922858 http://dx.doi.org/10.1371/journal.pone.0069927 Text en © 2013 Kumar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kumar, Dayanidhi Salian, Sujith Raj Kalthur, Guruprasad Uppangala, Shubhashree Kumari, Sandhya Challapalli, Srinivas Chandraguthi, Srinidhi Gururajarao Krishnamurthy, Hanumanthappa Jain, Navya Kumar, Pratap Adiga, Satish Kumar Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title | Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title_full | Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title_fullStr | Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title_full_unstemmed | Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title_short | Semen Abnormalities, Sperm DNA Damage and Global Hypermethylation in Health Workers Occupationally Exposed to Ionizing Radiation |
title_sort | semen abnormalities, sperm dna damage and global hypermethylation in health workers occupationally exposed to ionizing radiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726700/ https://www.ncbi.nlm.nih.gov/pubmed/23922858 http://dx.doi.org/10.1371/journal.pone.0069927 |
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