Cargando…

Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin

Envoplakin, periplakin and desmoplakin are cytoskeletal proteins that provide structural integrity within the skin and heart by resisting shear forces. Here we reveal the nature of unique hinges within their plakin domains that provides divergent degrees of flexibility between rigid long and short a...

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Jassar, Caezar, Bernadό, Pau, Chidgey, Martyn, Overduin, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726778/
https://www.ncbi.nlm.nih.gov/pubmed/23922795
http://dx.doi.org/10.1371/journal.pone.0069767
_version_ 1782278709683683328
author Al-Jassar, Caezar
Bernadό, Pau
Chidgey, Martyn
Overduin, Michael
author_facet Al-Jassar, Caezar
Bernadό, Pau
Chidgey, Martyn
Overduin, Michael
author_sort Al-Jassar, Caezar
collection PubMed
description Envoplakin, periplakin and desmoplakin are cytoskeletal proteins that provide structural integrity within the skin and heart by resisting shear forces. Here we reveal the nature of unique hinges within their plakin domains that provides divergent degrees of flexibility between rigid long and short arms composed of spectrin repeats. The range of mobility of the two arms about the hinge is revealed by applying the ensemble optimization method to small-angle X-ray scattering data. Envoplakin and periplakin adopt ‘L’ shaped conformations exhibiting a ‘helicopter propeller’-like mobility about the hinge. By contrast desmoplakin exhibits essentially unrestricted mobility by ‘jack-knifing’ about the hinge. Thus the diversity of molecular jointing that can occur about plakin hinges includes ‘L’ shaped bends, ‘U’ turns and fully extended ‘I’ orientations between rigid blocks of spectrin repeats. This establishes specialised hinges in plakin domains as a key source of flexibility that may allow sweeping of cellular spaces during assembly of cellular structures and could impart adaptability, so preventing irreversible damage to desmosomes and the cell cytoskeleton upon exposure to mechanical stress.
format Online
Article
Text
id pubmed-3726778
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-37267782013-08-06 Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin Al-Jassar, Caezar Bernadό, Pau Chidgey, Martyn Overduin, Michael PLoS One Research Article Envoplakin, periplakin and desmoplakin are cytoskeletal proteins that provide structural integrity within the skin and heart by resisting shear forces. Here we reveal the nature of unique hinges within their plakin domains that provides divergent degrees of flexibility between rigid long and short arms composed of spectrin repeats. The range of mobility of the two arms about the hinge is revealed by applying the ensemble optimization method to small-angle X-ray scattering data. Envoplakin and periplakin adopt ‘L’ shaped conformations exhibiting a ‘helicopter propeller’-like mobility about the hinge. By contrast desmoplakin exhibits essentially unrestricted mobility by ‘jack-knifing’ about the hinge. Thus the diversity of molecular jointing that can occur about plakin hinges includes ‘L’ shaped bends, ‘U’ turns and fully extended ‘I’ orientations between rigid blocks of spectrin repeats. This establishes specialised hinges in plakin domains as a key source of flexibility that may allow sweeping of cellular spaces during assembly of cellular structures and could impart adaptability, so preventing irreversible damage to desmosomes and the cell cytoskeleton upon exposure to mechanical stress. Public Library of Science 2013-07-29 /pmc/articles/PMC3726778/ /pubmed/23922795 http://dx.doi.org/10.1371/journal.pone.0069767 Text en © 2013 Al-Jassar et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Al-Jassar, Caezar
Bernadό, Pau
Chidgey, Martyn
Overduin, Michael
Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title_full Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title_fullStr Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title_full_unstemmed Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title_short Hinged Plakin Domains Provide Specialized Degrees of Articulation in Envoplakin, Periplakin and Desmoplakin
title_sort hinged plakin domains provide specialized degrees of articulation in envoplakin, periplakin and desmoplakin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3726778/
https://www.ncbi.nlm.nih.gov/pubmed/23922795
http://dx.doi.org/10.1371/journal.pone.0069767
work_keys_str_mv AT aljassarcaezar hingedplakindomainsprovidespecializeddegreesofarticulationinenvoplakinperiplakinanddesmoplakin
AT bernadopau hingedplakindomainsprovidespecializeddegreesofarticulationinenvoplakinperiplakinanddesmoplakin
AT chidgeymartyn hingedplakindomainsprovidespecializeddegreesofarticulationinenvoplakinperiplakinanddesmoplakin
AT overduinmichael hingedplakindomainsprovidespecializeddegreesofarticulationinenvoplakinperiplakinanddesmoplakin