Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells

Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasm...

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Autores principales: Gantke, Thorsten, Boussouf, Sabrina, Janzen, Julia, Morrice, Nicholas A., Howell, Steven, Mühlberger, Elke, Ley, Steven C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Accelerated Publication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727213/
https://www.ncbi.nlm.nih.gov/pubmed/23557442
http://dx.doi.org/10.1042/BJ20121873
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author Gantke, Thorsten
Boussouf, Sabrina
Janzen, Julia
Morrice, Nicholas A.
Howell, Steven
Mühlberger, Elke
Ley, Steven C.
author_facet Gantke, Thorsten
Boussouf, Sabrina
Janzen, Julia
Morrice, Nicholas A.
Howell, Steven
Mühlberger, Elke
Ley, Steven C.
author_sort Gantke, Thorsten
collection PubMed
description Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)–ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]–NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells.
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spelling pubmed-37272132013-08-06 Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells Gantke, Thorsten Boussouf, Sabrina Janzen, Julia Morrice, Nicholas A. Howell, Steven Mühlberger, Elke Ley, Steven C. Biochem J Accelerated Publication Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)–ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]–NF-κB1 p105 complex. VP35 also increased expression of other co-transfected proteins, suggesting that VP35 could be employed generally to boost recombinant protein production by HEK-293 cells. Portland Press Ltd. 2013-05-10 2013-06-01 /pmc/articles/PMC3727213/ /pubmed/23557442 http://dx.doi.org/10.1042/BJ20121873 Text en © 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Accelerated Publication
Gantke, Thorsten
Boussouf, Sabrina
Janzen, Julia
Morrice, Nicholas A.
Howell, Steven
Mühlberger, Elke
Ley, Steven C.
Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title_full Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title_fullStr Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title_full_unstemmed Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title_short Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells
title_sort ebola virus vp35 induces high-level production of recombinant tpl-2–abin-2–nf-κb1 p105 complex in co-transfected hek-293 cells
topic Accelerated Publication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727213/
https://www.ncbi.nlm.nih.gov/pubmed/23557442
http://dx.doi.org/10.1042/BJ20121873
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