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Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. The complex drives membrane scission events in a range of processes, including cytokinesis in Metazoa and some Archaea. CdvA is the protein in Archaea that recruits ESCRT-III to the m...

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Autores principales: Dobro, Megan J., Samson, Rachel Y., Yu, Zhiheng, McCullough, John, Ding, H. Jane, Chong, Parkson Lee-Gau, Bell, Stephen D., Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727925/
https://www.ncbi.nlm.nih.gov/pubmed/23761076
http://dx.doi.org/10.1091/mbc.E12-11-0785
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author Dobro, Megan J.
Samson, Rachel Y.
Yu, Zhiheng
McCullough, John
Ding, H. Jane
Chong, Parkson Lee-Gau
Bell, Stephen D.
Jensen, Grant J.
author_facet Dobro, Megan J.
Samson, Rachel Y.
Yu, Zhiheng
McCullough, John
Ding, H. Jane
Chong, Parkson Lee-Gau
Bell, Stephen D.
Jensen, Grant J.
author_sort Dobro, Megan J.
collection PubMed
description The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. The complex drives membrane scission events in a range of processes, including cytokinesis in Metazoa and some Archaea. CdvA is the protein in Archaea that recruits ESCRT-III to the membrane. Using electron cryotomography (ECT), we find that CdvA polymerizes into helical filaments wrapped around liposomes. ESCRT-III proteins are responsible for the cinching of membranes and have been shown to assemble into helical tubes in vitro, but here we show that they also can form nested tubes and nested cones, which reveal surprisingly numerous and versatile contacts. To observe the ESCRT–CdvA complex in a physiological context, we used ECT to image the archaeon Sulfolobus acidocaldarius and observed a distinct protein belt at the leading edge of constriction furrows in dividing cells. The known dimensions of ESCRT-III proteins constrain their possible orientations within each of these structures and point to the involvement of spiraling filaments in membrane scission.
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spelling pubmed-37279252013-10-16 Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission Dobro, Megan J. Samson, Rachel Y. Yu, Zhiheng McCullough, John Ding, H. Jane Chong, Parkson Lee-Gau Bell, Stephen D. Jensen, Grant J. Mol Biol Cell Articles The endosomal-sorting complex required for transport (ESCRT) is evolutionarily conserved from Archaea to eukaryotes. The complex drives membrane scission events in a range of processes, including cytokinesis in Metazoa and some Archaea. CdvA is the protein in Archaea that recruits ESCRT-III to the membrane. Using electron cryotomography (ECT), we find that CdvA polymerizes into helical filaments wrapped around liposomes. ESCRT-III proteins are responsible for the cinching of membranes and have been shown to assemble into helical tubes in vitro, but here we show that they also can form nested tubes and nested cones, which reveal surprisingly numerous and versatile contacts. To observe the ESCRT–CdvA complex in a physiological context, we used ECT to image the archaeon Sulfolobus acidocaldarius and observed a distinct protein belt at the leading edge of constriction furrows in dividing cells. The known dimensions of ESCRT-III proteins constrain their possible orientations within each of these structures and point to the involvement of spiraling filaments in membrane scission. The American Society for Cell Biology 2013-08-01 /pmc/articles/PMC3727925/ /pubmed/23761076 http://dx.doi.org/10.1091/mbc.E12-11-0785 Text en © 2013 Dobro et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Dobro, Megan J.
Samson, Rachel Y.
Yu, Zhiheng
McCullough, John
Ding, H. Jane
Chong, Parkson Lee-Gau
Bell, Stephen D.
Jensen, Grant J.
Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title_full Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title_fullStr Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title_full_unstemmed Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title_short Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission
title_sort electron cryotomography of escrt assemblies and dividing sulfolobus cells suggests that spiraling filaments are involved in membrane scission
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3727925/
https://www.ncbi.nlm.nih.gov/pubmed/23761076
http://dx.doi.org/10.1091/mbc.E12-11-0785
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