Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot

An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is ba...

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Autores principales: Vignon, Christine, Debeissat, Christelle, Georget, Marie-Thérèse, Bouscary, Didier, Gyan, Emmanuel, Rosset, Philippe, Herault, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728345/
https://www.ncbi.nlm.nih.gov/pubmed/23935867
http://dx.doi.org/10.1371/journal.pone.0068425
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author Vignon, Christine
Debeissat, Christelle
Georget, Marie-Thérèse
Bouscary, Didier
Gyan, Emmanuel
Rosset, Philippe
Herault, Olivier
author_facet Vignon, Christine
Debeissat, Christelle
Georget, Marie-Thérèse
Bouscary, Didier
Gyan, Emmanuel
Rosset, Philippe
Herault, Olivier
author_sort Vignon, Christine
collection PubMed
description An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.
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spelling pubmed-37283452013-08-09 Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot Vignon, Christine Debeissat, Christelle Georget, Marie-Thérèse Bouscary, Didier Gyan, Emmanuel Rosset, Philippe Herault, Olivier PLoS One Research Article An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers. Public Library of Science 2013-07-30 /pmc/articles/PMC3728345/ /pubmed/23935867 http://dx.doi.org/10.1371/journal.pone.0068425 Text en © 2013 Vignon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Vignon, Christine
Debeissat, Christelle
Georget, Marie-Thérèse
Bouscary, Didier
Gyan, Emmanuel
Rosset, Philippe
Herault, Olivier
Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title_full Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title_fullStr Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title_full_unstemmed Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title_short Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-Color Fluorescence Plot
title_sort flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728345/
https://www.ncbi.nlm.nih.gov/pubmed/23935867
http://dx.doi.org/10.1371/journal.pone.0068425
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