Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes

The protozoan parasites of the genus Leishmania are the causative agents of different clinical diseases. Fetal calf serum (FCS) is the main part and the most expensive ingredient of the Leishmania culture media. Here, the efficacies of different concentrations (1%, 2.5%, 5%, and 10%) of the filtered...

Descripción completa

Detalles Bibliográficos
Autores principales: Nasiri, Vahid, Karimi, Gholamreza, Dalimi, Abdolhossein, Paykari, Habibollah, Ghaffarifar, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728512/
https://www.ncbi.nlm.nih.gov/pubmed/23957003
http://dx.doi.org/10.1155/2013/748592
_version_ 1782278873038192640
author Nasiri, Vahid
Karimi, Gholamreza
Dalimi, Abdolhossein
Paykari, Habibollah
Ghaffarifar, Fatemeh
author_facet Nasiri, Vahid
Karimi, Gholamreza
Dalimi, Abdolhossein
Paykari, Habibollah
Ghaffarifar, Fatemeh
author_sort Nasiri, Vahid
collection PubMed
description The protozoan parasites of the genus Leishmania are the causative agents of different clinical diseases. Fetal calf serum (FCS) is the main part and the most expensive ingredient of the Leishmania culture media. Here, the efficacies of different concentrations (1%, 2.5%, 5%, and 10%) of the filtered and autoclaved sheep and mouse urine were evaluated as a growth stimulator in Leishmania culture procedure. The results indicated that culture media enriched with the filtered sheep and mouse urine supported the growth of the parasites and can be used for cultivation of Leishmania parasites. In conclusion, this study has demonstrated an alternative low-cost medium that could be used in cultivation process of Leishmania major promastigotes.
format Online
Article
Text
id pubmed-3728512
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37285122013-08-16 Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes Nasiri, Vahid Karimi, Gholamreza Dalimi, Abdolhossein Paykari, Habibollah Ghaffarifar, Fatemeh Biomed Res Int Research Article The protozoan parasites of the genus Leishmania are the causative agents of different clinical diseases. Fetal calf serum (FCS) is the main part and the most expensive ingredient of the Leishmania culture media. Here, the efficacies of different concentrations (1%, 2.5%, 5%, and 10%) of the filtered and autoclaved sheep and mouse urine were evaluated as a growth stimulator in Leishmania culture procedure. The results indicated that culture media enriched with the filtered sheep and mouse urine supported the growth of the parasites and can be used for cultivation of Leishmania parasites. In conclusion, this study has demonstrated an alternative low-cost medium that could be used in cultivation process of Leishmania major promastigotes. Hindawi Publishing Corporation 2013 2013-07-15 /pmc/articles/PMC3728512/ /pubmed/23957003 http://dx.doi.org/10.1155/2013/748592 Text en Copyright © 2013 Vahid Nasiri et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nasiri, Vahid
Karimi, Gholamreza
Dalimi, Abdolhossein
Paykari, Habibollah
Ghaffarifar, Fatemeh
Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title_full Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title_fullStr Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title_full_unstemmed Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title_short Effects of Sheep and Mouse Urine on the Growth Pattern of Leishmania major Promastigotes
title_sort effects of sheep and mouse urine on the growth pattern of leishmania major promastigotes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728512/
https://www.ncbi.nlm.nih.gov/pubmed/23957003
http://dx.doi.org/10.1155/2013/748592
work_keys_str_mv AT nasirivahid effectsofsheepandmouseurineonthegrowthpatternofleishmaniamajorpromastigotes
AT karimigholamreza effectsofsheepandmouseurineonthegrowthpatternofleishmaniamajorpromastigotes
AT dalimiabdolhossein effectsofsheepandmouseurineonthegrowthpatternofleishmaniamajorpromastigotes
AT paykarihabibollah effectsofsheepandmouseurineonthegrowthpatternofleishmaniamajorpromastigotes
AT ghaffarifarfatemeh effectsofsheepandmouseurineonthegrowthpatternofleishmaniamajorpromastigotes

Ejemplares similares