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Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA),...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728601/ https://www.ncbi.nlm.nih.gov/pubmed/23900274 http://dx.doi.org/10.1038/srep02332 |
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author | Zhang, Weijia Choi, Dong Soon Nguyen, Yen H. Chang, Jenny Qin, Lidong |
author_facet | Zhang, Weijia Choi, Dong Soon Nguyen, Yen H. Chang, Jenny Qin, Lidong |
author_sort | Zhang, Weijia |
collection | PubMed |
description | This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA), or collagen with or without oxygen-plasma treatments of the PDMS surfaces dramatically impacted the phenotypic equilibrium of breast cancer stem cells, while the variations of the PDMS elastic stiffness had much less such effects. Our results showed that the surface coatings of collagen and fibronectin on PDMS maintained breast cancer cell phenotypes to be nearly identical to the cultures on commercial polystyrene Petri dishes. The surface coating of BSA provided a weak cell-substrate adhesion that stimulated the increase in stem-cell-like subpopulation. Our observations may potentially guide surface modification approaches to obtain specific cell phenotypes. |
format | Online Article Text |
id | pubmed-3728601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-37286012013-07-31 Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design Zhang, Weijia Choi, Dong Soon Nguyen, Yen H. Chang, Jenny Qin, Lidong Sci Rep Article This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA), or collagen with or without oxygen-plasma treatments of the PDMS surfaces dramatically impacted the phenotypic equilibrium of breast cancer stem cells, while the variations of the PDMS elastic stiffness had much less such effects. Our results showed that the surface coatings of collagen and fibronectin on PDMS maintained breast cancer cell phenotypes to be nearly identical to the cultures on commercial polystyrene Petri dishes. The surface coating of BSA provided a weak cell-substrate adhesion that stimulated the increase in stem-cell-like subpopulation. Our observations may potentially guide surface modification approaches to obtain specific cell phenotypes. Nature Publishing Group 2013-07-31 /pmc/articles/PMC3728601/ /pubmed/23900274 http://dx.doi.org/10.1038/srep02332 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Zhang, Weijia Choi, Dong Soon Nguyen, Yen H. Chang, Jenny Qin, Lidong Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title | Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title_full | Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title_fullStr | Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title_full_unstemmed | Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title_short | Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design |
title_sort | studying cancer stem cell dynamics on pdms surfaces for microfluidics device design |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728601/ https://www.ncbi.nlm.nih.gov/pubmed/23900274 http://dx.doi.org/10.1038/srep02332 |
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