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Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design

This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA),...

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Detalles Bibliográficos
Autores principales: Zhang, Weijia, Choi, Dong Soon, Nguyen, Yen H., Chang, Jenny, Qin, Lidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728601/
https://www.ncbi.nlm.nih.gov/pubmed/23900274
http://dx.doi.org/10.1038/srep02332
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author Zhang, Weijia
Choi, Dong Soon
Nguyen, Yen H.
Chang, Jenny
Qin, Lidong
author_facet Zhang, Weijia
Choi, Dong Soon
Nguyen, Yen H.
Chang, Jenny
Qin, Lidong
author_sort Zhang, Weijia
collection PubMed
description This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA), or collagen with or without oxygen-plasma treatments of the PDMS surfaces dramatically impacted the phenotypic equilibrium of breast cancer stem cells, while the variations of the PDMS elastic stiffness had much less such effects. Our results showed that the surface coatings of collagen and fibronectin on PDMS maintained breast cancer cell phenotypes to be nearly identical to the cultures on commercial polystyrene Petri dishes. The surface coating of BSA provided a weak cell-substrate adhesion that stimulated the increase in stem-cell-like subpopulation. Our observations may potentially guide surface modification approaches to obtain specific cell phenotypes.
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spelling pubmed-37286012013-07-31 Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design Zhang, Weijia Choi, Dong Soon Nguyen, Yen H. Chang, Jenny Qin, Lidong Sci Rep Article This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA), or collagen with or without oxygen-plasma treatments of the PDMS surfaces dramatically impacted the phenotypic equilibrium of breast cancer stem cells, while the variations of the PDMS elastic stiffness had much less such effects. Our results showed that the surface coatings of collagen and fibronectin on PDMS maintained breast cancer cell phenotypes to be nearly identical to the cultures on commercial polystyrene Petri dishes. The surface coating of BSA provided a weak cell-substrate adhesion that stimulated the increase in stem-cell-like subpopulation. Our observations may potentially guide surface modification approaches to obtain specific cell phenotypes. Nature Publishing Group 2013-07-31 /pmc/articles/PMC3728601/ /pubmed/23900274 http://dx.doi.org/10.1038/srep02332 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Zhang, Weijia
Choi, Dong Soon
Nguyen, Yen H.
Chang, Jenny
Qin, Lidong
Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title_full Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title_fullStr Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title_full_unstemmed Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title_short Studying Cancer Stem Cell Dynamics on PDMS Surfaces for Microfluidics Device Design
title_sort studying cancer stem cell dynamics on pdms surfaces for microfluidics device design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3728601/
https://www.ncbi.nlm.nih.gov/pubmed/23900274
http://dx.doi.org/10.1038/srep02332
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