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BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals

In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrate the expression of BAG3 protein both in equine sarcoids in vivo and in EqS04b cells, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome. Evidence...

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Autores principales: Cotugno, Roberta, Gallotta, Dario, d’Avenia, Morena, Corteggio, Annunziata, Altamura, Gennaro, Roperto, Franco, Belisario, Maria Antonietta, Borzacchiello, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729419/
https://www.ncbi.nlm.nih.gov/pubmed/23876161
http://dx.doi.org/10.1186/1297-9716-44-61
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author Cotugno, Roberta
Gallotta, Dario
d’Avenia, Morena
Corteggio, Annunziata
Altamura, Gennaro
Roperto, Franco
Belisario, Maria Antonietta
Borzacchiello, Giuseppe
author_facet Cotugno, Roberta
Gallotta, Dario
d’Avenia, Morena
Corteggio, Annunziata
Altamura, Gennaro
Roperto, Franco
Belisario, Maria Antonietta
Borzacchiello, Giuseppe
author_sort Cotugno, Roberta
collection PubMed
description In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrate the expression of BAG3 protein both in equine sarcoids in vivo and in EqS04b cells, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome. Evidence of a possible involvement of BAG3 in equine sarcoid carcinogenesis was obtained by immunohistochemistry analysis of tumour samples. We found that most tumour samples stained positive for BAG3, even though to a different grade, while normal dermal fibroblasts from healthy horses displayed very weak staining pattern for BAG3 expression. By siRNA technology, we demonstrate in EqS04b the role of BAG3 in counteracting basal as well as chemical-triggered pro-death signals. BAG3 down-modulation was indeed shown to promote cell death and cell cycle arrest in G(0)/G(1). In addition, we found that BAG3 silencing sensitized EqS04b cells to phenethylisothiocyanate (PEITC), a promising cancer chemopreventive/chemotherapeutic agent present in edible cruciferous vegetables. Notably, such a pro-survival role of BAG3 was less marked in E. Derm cells, an equine BPV-negative fibroblast cell line taken as a normal counterpart. Altogether our findings might suggest a mutual cooperation between BAG3 and viral oncoproteins to sustain cell survival.
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spelling pubmed-37294192013-08-01 BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals Cotugno, Roberta Gallotta, Dario d’Avenia, Morena Corteggio, Annunziata Altamura, Gennaro Roperto, Franco Belisario, Maria Antonietta Borzacchiello, Giuseppe Vet Res Research In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrate the expression of BAG3 protein both in equine sarcoids in vivo and in EqS04b cells, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome. Evidence of a possible involvement of BAG3 in equine sarcoid carcinogenesis was obtained by immunohistochemistry analysis of tumour samples. We found that most tumour samples stained positive for BAG3, even though to a different grade, while normal dermal fibroblasts from healthy horses displayed very weak staining pattern for BAG3 expression. By siRNA technology, we demonstrate in EqS04b the role of BAG3 in counteracting basal as well as chemical-triggered pro-death signals. BAG3 down-modulation was indeed shown to promote cell death and cell cycle arrest in G(0)/G(1). In addition, we found that BAG3 silencing sensitized EqS04b cells to phenethylisothiocyanate (PEITC), a promising cancer chemopreventive/chemotherapeutic agent present in edible cruciferous vegetables. Notably, such a pro-survival role of BAG3 was less marked in E. Derm cells, an equine BPV-negative fibroblast cell line taken as a normal counterpart. Altogether our findings might suggest a mutual cooperation between BAG3 and viral oncoproteins to sustain cell survival. BioMed Central 2013 2013-07-22 /pmc/articles/PMC3729419/ /pubmed/23876161 http://dx.doi.org/10.1186/1297-9716-44-61 Text en Copyright © 2013 Cotugno et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Cotugno, Roberta
Gallotta, Dario
d’Avenia, Morena
Corteggio, Annunziata
Altamura, Gennaro
Roperto, Franco
Belisario, Maria Antonietta
Borzacchiello, Giuseppe
BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title_full BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title_fullStr BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title_full_unstemmed BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title_short BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals
title_sort bag3 protects bovine papillomavirus type 1-transformed equine fibroblasts against pro-death signals
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729419/
https://www.ncbi.nlm.nih.gov/pubmed/23876161
http://dx.doi.org/10.1186/1297-9716-44-61
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