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Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function
Myosin binding protein-C (MyBP-C) exists in three major isoforms: slow skeletal, fast skeletal, and cardiac. While cardiac MyBP-C (cMyBP-C) expression is restricted to the heart in the adult, it is transiently expressed in neonatal stages of some skeletal muscles. However, it is unclear whether this...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729691/ https://www.ncbi.nlm.nih.gov/pubmed/23936073 http://dx.doi.org/10.1371/journal.pone.0069671 |
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author | Lin, Brian Govindan, Suresh Lee, Kyounghwan Zhao, Piming Han, Renzhi Runte, K. Elisabeth Craig, Roger Palmer, Bradley M. Sadayappan, Sakthivel |
author_facet | Lin, Brian Govindan, Suresh Lee, Kyounghwan Zhao, Piming Han, Renzhi Runte, K. Elisabeth Craig, Roger Palmer, Bradley M. Sadayappan, Sakthivel |
author_sort | Lin, Brian |
collection | PubMed |
description | Myosin binding protein-C (MyBP-C) exists in three major isoforms: slow skeletal, fast skeletal, and cardiac. While cardiac MyBP-C (cMyBP-C) expression is restricted to the heart in the adult, it is transiently expressed in neonatal stages of some skeletal muscles. However, it is unclear whether this expression is necessary for the proper development and function of skeletal muscle. Our aim was to determine whether the absence of cMyBP-C alters the structure, function, or MyBP-C isoform expression in adult skeletal muscle using a cMyBP-C null mouse model (cMyBP-C((t/t))). Slow MyBP-C was expressed in both slow and fast skeletal muscles, whereas fast MyBP-C was mostly restricted to fast skeletal muscles. Expression of these isoforms was unaffected in skeletal muscle from cMyBP-C((t/t)) mice. Slow and fast skeletal muscles in cMyBP-C((t/t)) mice showed no histological or ultrastructural changes in comparison to the wild-type control. In addition, slow muscle twitch, tetanus tension, and susceptibility to injury were all similar to the wild-type controls. Interestingly, fMyBP-C expression was significantly increased in the cMyBP-C((t/t)) hearts undergoing severe dilated cardiomyopathy, though this does not seem to prevent dysfunction. Additionally, expression of both slow and fast isoforms was increased in myopathic skeletal muscles. Our data demonstrate that i) MyBP-C isoforms are differentially regulated in both cardiac and skeletal muscles, ii) cMyBP-C is dispensable for the development of skeletal muscle with no functional or structural consequences in the adult myocyte, and iii) skeletal isoforms can transcomplement in the heart in the absence of cMyBP-C. |
format | Online Article Text |
id | pubmed-3729691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37296912013-08-09 Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function Lin, Brian Govindan, Suresh Lee, Kyounghwan Zhao, Piming Han, Renzhi Runte, K. Elisabeth Craig, Roger Palmer, Bradley M. Sadayappan, Sakthivel PLoS One Research Article Myosin binding protein-C (MyBP-C) exists in three major isoforms: slow skeletal, fast skeletal, and cardiac. While cardiac MyBP-C (cMyBP-C) expression is restricted to the heart in the adult, it is transiently expressed in neonatal stages of some skeletal muscles. However, it is unclear whether this expression is necessary for the proper development and function of skeletal muscle. Our aim was to determine whether the absence of cMyBP-C alters the structure, function, or MyBP-C isoform expression in adult skeletal muscle using a cMyBP-C null mouse model (cMyBP-C((t/t))). Slow MyBP-C was expressed in both slow and fast skeletal muscles, whereas fast MyBP-C was mostly restricted to fast skeletal muscles. Expression of these isoforms was unaffected in skeletal muscle from cMyBP-C((t/t)) mice. Slow and fast skeletal muscles in cMyBP-C((t/t)) mice showed no histological or ultrastructural changes in comparison to the wild-type control. In addition, slow muscle twitch, tetanus tension, and susceptibility to injury were all similar to the wild-type controls. Interestingly, fMyBP-C expression was significantly increased in the cMyBP-C((t/t)) hearts undergoing severe dilated cardiomyopathy, though this does not seem to prevent dysfunction. Additionally, expression of both slow and fast isoforms was increased in myopathic skeletal muscles. Our data demonstrate that i) MyBP-C isoforms are differentially regulated in both cardiac and skeletal muscles, ii) cMyBP-C is dispensable for the development of skeletal muscle with no functional or structural consequences in the adult myocyte, and iii) skeletal isoforms can transcomplement in the heart in the absence of cMyBP-C. Public Library of Science 2013-07-31 /pmc/articles/PMC3729691/ /pubmed/23936073 http://dx.doi.org/10.1371/journal.pone.0069671 Text en © 2013 Lin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lin, Brian Govindan, Suresh Lee, Kyounghwan Zhao, Piming Han, Renzhi Runte, K. Elisabeth Craig, Roger Palmer, Bradley M. Sadayappan, Sakthivel Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title | Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title_full | Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title_fullStr | Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title_full_unstemmed | Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title_short | Cardiac Myosin Binding Protein-C Plays No Regulatory Role in Skeletal Muscle Structure and Function |
title_sort | cardiac myosin binding protein-c plays no regulatory role in skeletal muscle structure and function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729691/ https://www.ncbi.nlm.nih.gov/pubmed/23936073 http://dx.doi.org/10.1371/journal.pone.0069671 |
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