Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and a...

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Autores principales: Kallon, Sanpha, Li, Xiaorong, Ji, Jun, Chen, Cuiying, Xi, Qianyun, Chang, Shuang, Xue, Chunyi, Ma, Jingyun, Xie, Qingmei, Zhang, Youngliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729712/
https://www.ncbi.nlm.nih.gov/pubmed/23786718
http://dx.doi.org/10.1186/2049-1891-4-22
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author Kallon, Sanpha
Li, Xiaorong
Ji, Jun
Chen, Cuiying
Xi, Qianyun
Chang, Shuang
Xue, Chunyi
Ma, Jingyun
Xie, Qingmei
Zhang, Youngliang
author_facet Kallon, Sanpha
Li, Xiaorong
Ji, Jun
Chen, Cuiying
Xi, Qianyun
Chang, Shuang
Xue, Chunyi
Ma, Jingyun
Xie, Qingmei
Zhang, Youngliang
author_sort Kallon, Sanpha
collection PubMed
description This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.
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spelling pubmed-37297122013-08-01 Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo Kallon, Sanpha Li, Xiaorong Ji, Jun Chen, Cuiying Xi, Qianyun Chang, Shuang Xue, Chunyi Ma, Jingyun Xie, Qingmei Zhang, Youngliang J Anim Sci Biotechnol Research This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens. BioMed Central 2013-06-21 /pmc/articles/PMC3729712/ /pubmed/23786718 http://dx.doi.org/10.1186/2049-1891-4-22 Text en Copyright © 2013 Kallon et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kallon, Sanpha
Li, Xiaorong
Ji, Jun
Chen, Cuiying
Xi, Qianyun
Chang, Shuang
Xue, Chunyi
Ma, Jingyun
Xie, Qingmei
Zhang, Youngliang
Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title_full Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title_fullStr Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title_full_unstemmed Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title_short Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo
title_sort astragalus polysaccharide enhances immunity and inhibits h9n2 avian influenza virus in vitro and in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729712/
https://www.ncbi.nlm.nih.gov/pubmed/23786718
http://dx.doi.org/10.1186/2049-1891-4-22
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