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Clinical Significance of CK19 Negative Breast Cancer

Analysis of sentinel lymph nodes (SLNs) by means of One-Step Nucleic Acid Amplification (OSNA) is gaining widespread use as a quick and accurate method. This assay detects the expression level of cytokeratin 19 (CK19) which is present in some but not all breast tumors. In this study, the clinical si...

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Autores principales: Fujisue, Mamiko, Nishimura, Reiki, Okumura, Yasuhiro, Tashima, Rumiko, Nishiyama, Yasuyuki, Osako, Tomofumi, Toyozumi, Yasuo, Arima, Nobuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730313/
https://www.ncbi.nlm.nih.gov/pubmed/24216695
http://dx.doi.org/10.3390/cancers5010001
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author Fujisue, Mamiko
Nishimura, Reiki
Okumura, Yasuhiro
Tashima, Rumiko
Nishiyama, Yasuyuki
Osako, Tomofumi
Toyozumi, Yasuo
Arima, Nobuyuki
author_facet Fujisue, Mamiko
Nishimura, Reiki
Okumura, Yasuhiro
Tashima, Rumiko
Nishiyama, Yasuyuki
Osako, Tomofumi
Toyozumi, Yasuo
Arima, Nobuyuki
author_sort Fujisue, Mamiko
collection PubMed
description Analysis of sentinel lymph nodes (SLNs) by means of One-Step Nucleic Acid Amplification (OSNA) is gaining widespread use as a quick and accurate method. This assay detects the expression level of cytokeratin 19 (CK19) which is present in some but not all breast tumors. In this study, the clinical significance of negative CK19 was investigated in 219 cases of primary breast cancer. In 179 patients with clinically negative nodes, OSNA and imprint smear cytology of SLN were performed simultaneously. The OSNA revealed a node-positive rate of 24.6%. Negative CK19 correlated significantly with negative ER/PgR and higher Ki-67 values, and marginally with higher nuclear grade and p53 overexpression. The triple negative subtype showed lower CK19 expression. OSNA revealed that one of the negative CK19 cases was actually a false negative but this was corrected with the use of the imprint smear cytology. In conclusion, CK19 negativity reflected the aggressiveness of primary breast cancer. OSNA assay used to analyze SLN was useful, but there is a possibility that it will mistakenly detect false negatives in CK19 negative tumors. Therefore, in tumors with negative CK19, the imprint smear cytology may be more useful in cases with macrometastasis.
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spelling pubmed-37303132013-08-05 Clinical Significance of CK19 Negative Breast Cancer Fujisue, Mamiko Nishimura, Reiki Okumura, Yasuhiro Tashima, Rumiko Nishiyama, Yasuyuki Osako, Tomofumi Toyozumi, Yasuo Arima, Nobuyuki Cancers (Basel) Article Analysis of sentinel lymph nodes (SLNs) by means of One-Step Nucleic Acid Amplification (OSNA) is gaining widespread use as a quick and accurate method. This assay detects the expression level of cytokeratin 19 (CK19) which is present in some but not all breast tumors. In this study, the clinical significance of negative CK19 was investigated in 219 cases of primary breast cancer. In 179 patients with clinically negative nodes, OSNA and imprint smear cytology of SLN were performed simultaneously. The OSNA revealed a node-positive rate of 24.6%. Negative CK19 correlated significantly with negative ER/PgR and higher Ki-67 values, and marginally with higher nuclear grade and p53 overexpression. The triple negative subtype showed lower CK19 expression. OSNA revealed that one of the negative CK19 cases was actually a false negative but this was corrected with the use of the imprint smear cytology. In conclusion, CK19 negativity reflected the aggressiveness of primary breast cancer. OSNA assay used to analyze SLN was useful, but there is a possibility that it will mistakenly detect false negatives in CK19 negative tumors. Therefore, in tumors with negative CK19, the imprint smear cytology may be more useful in cases with macrometastasis. MDPI 2012-12-21 /pmc/articles/PMC3730313/ /pubmed/24216695 http://dx.doi.org/10.3390/cancers5010001 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Fujisue, Mamiko
Nishimura, Reiki
Okumura, Yasuhiro
Tashima, Rumiko
Nishiyama, Yasuyuki
Osako, Tomofumi
Toyozumi, Yasuo
Arima, Nobuyuki
Clinical Significance of CK19 Negative Breast Cancer
title Clinical Significance of CK19 Negative Breast Cancer
title_full Clinical Significance of CK19 Negative Breast Cancer
title_fullStr Clinical Significance of CK19 Negative Breast Cancer
title_full_unstemmed Clinical Significance of CK19 Negative Breast Cancer
title_short Clinical Significance of CK19 Negative Breast Cancer
title_sort clinical significance of ck19 negative breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730313/
https://www.ncbi.nlm.nih.gov/pubmed/24216695
http://dx.doi.org/10.3390/cancers5010001
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