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Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and sub...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730329/ https://www.ncbi.nlm.nih.gov/pubmed/24216982 http://dx.doi.org/10.3390/cancers5020372 |
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author | Goudarzi, Atta Gokgoz, Nalan Gill, Mona Pinnaduwage, Dushanthi Merico, Daniele Wunder, Jay S. Andrulis, Irene L. |
author_facet | Goudarzi, Atta Gokgoz, Nalan Gill, Mona Pinnaduwage, Dushanthi Merico, Daniele Wunder, Jay S. Andrulis, Irene L. |
author_sort | Goudarzi, Atta |
collection | PubMed |
description | Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and subsequent supervised network analysis, was performed to discover genetic networks differentially activated or organized in metastatic OS compared to localized OS. Broad trends among the profiles of metastatic tumors include aberrant activity of intracellular organization and translation networks, as well as disorganization of metabolic networks. The differentially activated PRKCε-RASGRP3-GNB2 network, which interacts with the disorganized DLG2 hub, was also found to be differentially expressed among OS cell lines with differing metastatic capacity in xenograft models. PRKCε transcript was more abundant in some metastatic OS tumors; however the difference was not significant overall. In functional studies, PRKCε was not found to be involved in migration of M132 OS cells, but its protein expression was induced in M112 OS cells following IGF-1 stimulation. |
format | Online Article Text |
id | pubmed-3730329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-37303292013-08-05 Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis Goudarzi, Atta Gokgoz, Nalan Gill, Mona Pinnaduwage, Dushanthi Merico, Daniele Wunder, Jay S. Andrulis, Irene L. Cancers (Basel) Article Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and subsequent supervised network analysis, was performed to discover genetic networks differentially activated or organized in metastatic OS compared to localized OS. Broad trends among the profiles of metastatic tumors include aberrant activity of intracellular organization and translation networks, as well as disorganization of metabolic networks. The differentially activated PRKCε-RASGRP3-GNB2 network, which interacts with the disorganized DLG2 hub, was also found to be differentially expressed among OS cell lines with differing metastatic capacity in xenograft models. PRKCε transcript was more abundant in some metastatic OS tumors; however the difference was not significant overall. In functional studies, PRKCε was not found to be involved in migration of M132 OS cells, but its protein expression was induced in M112 OS cells following IGF-1 stimulation. MDPI 2013-04-08 /pmc/articles/PMC3730329/ /pubmed/24216982 http://dx.doi.org/10.3390/cancers5020372 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Goudarzi, Atta Gokgoz, Nalan Gill, Mona Pinnaduwage, Dushanthi Merico, Daniele Wunder, Jay S. Andrulis, Irene L. Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title | Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title_full | Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title_fullStr | Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title_full_unstemmed | Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title_short | Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis |
title_sort | protein kinase c epsilon and genetic networks in osteosarcoma metastasis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730329/ https://www.ncbi.nlm.nih.gov/pubmed/24216982 http://dx.doi.org/10.3390/cancers5020372 |
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