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Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis

Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and sub...

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Autores principales: Goudarzi, Atta, Gokgoz, Nalan, Gill, Mona, Pinnaduwage, Dushanthi, Merico, Daniele, Wunder, Jay S., Andrulis, Irene L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730329/
https://www.ncbi.nlm.nih.gov/pubmed/24216982
http://dx.doi.org/10.3390/cancers5020372
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author Goudarzi, Atta
Gokgoz, Nalan
Gill, Mona
Pinnaduwage, Dushanthi
Merico, Daniele
Wunder, Jay S.
Andrulis, Irene L.
author_facet Goudarzi, Atta
Gokgoz, Nalan
Gill, Mona
Pinnaduwage, Dushanthi
Merico, Daniele
Wunder, Jay S.
Andrulis, Irene L.
author_sort Goudarzi, Atta
collection PubMed
description Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and subsequent supervised network analysis, was performed to discover genetic networks differentially activated or organized in metastatic OS compared to localized OS. Broad trends among the profiles of metastatic tumors include aberrant activity of intracellular organization and translation networks, as well as disorganization of metabolic networks. The differentially activated PRKCε-RASGRP3-GNB2 network, which interacts with the disorganized DLG2 hub, was also found to be differentially expressed among OS cell lines with differing metastatic capacity in xenograft models. PRKCε transcript was more abundant in some metastatic OS tumors; however the difference was not significant overall. In functional studies, PRKCε was not found to be involved in migration of M132 OS cells, but its protein expression was induced in M112 OS cells following IGF-1 stimulation.
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spelling pubmed-37303292013-08-05 Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis Goudarzi, Atta Gokgoz, Nalan Gill, Mona Pinnaduwage, Dushanthi Merico, Daniele Wunder, Jay S. Andrulis, Irene L. Cancers (Basel) Article Osteosarcoma (OS) is the most common primary malignant tumor of the bone, and pulmonary metastasis is the most frequent cause of OS mortality. The aim of this study was to discover and characterize genetic networks differentially expressed in metastatic OS. Expression profiling of OS tumors, and subsequent supervised network analysis, was performed to discover genetic networks differentially activated or organized in metastatic OS compared to localized OS. Broad trends among the profiles of metastatic tumors include aberrant activity of intracellular organization and translation networks, as well as disorganization of metabolic networks. The differentially activated PRKCε-RASGRP3-GNB2 network, which interacts with the disorganized DLG2 hub, was also found to be differentially expressed among OS cell lines with differing metastatic capacity in xenograft models. PRKCε transcript was more abundant in some metastatic OS tumors; however the difference was not significant overall. In functional studies, PRKCε was not found to be involved in migration of M132 OS cells, but its protein expression was induced in M112 OS cells following IGF-1 stimulation. MDPI 2013-04-08 /pmc/articles/PMC3730329/ /pubmed/24216982 http://dx.doi.org/10.3390/cancers5020372 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Goudarzi, Atta
Gokgoz, Nalan
Gill, Mona
Pinnaduwage, Dushanthi
Merico, Daniele
Wunder, Jay S.
Andrulis, Irene L.
Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title_full Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title_fullStr Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title_full_unstemmed Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title_short Protein Kinase C Epsilon and Genetic Networks in Osteosarcoma Metastasis
title_sort protein kinase c epsilon and genetic networks in osteosarcoma metastasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730329/
https://www.ncbi.nlm.nih.gov/pubmed/24216982
http://dx.doi.org/10.3390/cancers5020372
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