Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene fun...

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Detalles Bibliográficos
Autores principales: Gratz, Scott J., Cummings, Alexander M., Nguyen, Jennifer N., Hamm, Danielle C., Donohue, Laura K., Harrison, Melissa M., Wildonger, Jill, O’Connor-Giles, Kate M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730909/
https://www.ncbi.nlm.nih.gov/pubmed/23709638
http://dx.doi.org/10.1534/genetics.113.152710
Descripción
Sumario:We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

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