Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene fun...

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Detalles Bibliográficos
Autores principales: Gratz, Scott J., Cummings, Alexander M., Nguyen, Jennifer N., Hamm, Danielle C., Donohue, Laura K., Harrison, Melissa M., Wildonger, Jill, O’Connor-Giles, Kate M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730909/
https://www.ncbi.nlm.nih.gov/pubmed/23709638
http://dx.doi.org/10.1534/genetics.113.152710
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author Gratz, Scott J.
Cummings, Alexander M.
Nguyen, Jennifer N.
Hamm, Danielle C.
Donohue, Laura K.
Harrison, Melissa M.
Wildonger, Jill
O’Connor-Giles, Kate M.
author_facet Gratz, Scott J.
Cummings, Alexander M.
Nguyen, Jennifer N.
Hamm, Danielle C.
Donohue, Laura K.
Harrison, Melissa M.
Wildonger, Jill
O’Connor-Giles, Kate M.
author_sort Gratz, Scott J.
collection PubMed
description We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
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spelling pubmed-37309092013-08-01 Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease Gratz, Scott J. Cummings, Alexander M. Nguyen, Jennifer N. Hamm, Danielle C. Donohue, Laura K. Harrison, Melissa M. Wildonger, Jill O’Connor-Giles, Kate M. Genetics Notes We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila. Genetics Society of America 2013-08 /pmc/articles/PMC3730909/ /pubmed/23709638 http://dx.doi.org/10.1534/genetics.113.152710 Text en Copyright © 2013 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle Notes
Gratz, Scott J.
Cummings, Alexander M.
Nguyen, Jennifer N.
Hamm, Danielle C.
Donohue, Laura K.
Harrison, Melissa M.
Wildonger, Jill
O’Connor-Giles, Kate M.
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title_full Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title_fullStr Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title_full_unstemmed Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title_short Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
title_sort genome engineering of drosophila with the crispr rna-guided cas9 nuclease
topic Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730909/
https://www.ncbi.nlm.nih.gov/pubmed/23709638
http://dx.doi.org/10.1534/genetics.113.152710
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