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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene fun...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730909/ https://www.ncbi.nlm.nih.gov/pubmed/23709638 http://dx.doi.org/10.1534/genetics.113.152710 |