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Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils

Cytosolic lipid droplets (LDs), which are now recognized as multifunctional organelles, accumulate in leukocytes under various inflammatory conditions. However, little is known about the characteristic features of LDs in neutrophils. In this study, we show that perilipin-3 (PLIN3; formerly called TI...

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Autores principales: Nose, Fuyuki, Yamaguchi, Tomohiro, Kato, Rina, Aiuchi, Toshihiro, Obama, Takashi, Hara, Shuntaro, Yamamoto, Matsuo, Itabe, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731282/
https://www.ncbi.nlm.nih.gov/pubmed/23936516
http://dx.doi.org/10.1371/journal.pone.0071542
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author Nose, Fuyuki
Yamaguchi, Tomohiro
Kato, Rina
Aiuchi, Toshihiro
Obama, Takashi
Hara, Shuntaro
Yamamoto, Matsuo
Itabe, Hiroyuki
author_facet Nose, Fuyuki
Yamaguchi, Tomohiro
Kato, Rina
Aiuchi, Toshihiro
Obama, Takashi
Hara, Shuntaro
Yamamoto, Matsuo
Itabe, Hiroyuki
author_sort Nose, Fuyuki
collection PubMed
description Cytosolic lipid droplets (LDs), which are now recognized as multifunctional organelles, accumulate in leukocytes under various inflammatory conditions. However, little is known about the characteristic features of LDs in neutrophils. In this study, we show that perilipin-3 (PLIN3; formerly called TIP47) is involved in LD formation and the inflammatory response in HL-60-derived neutrophils. HL-60, a promyelocytic cell line, was differentiated into neutrophils via treatment with all-trans retinoic acid. After differentiation, cells were stimulated with Porphyromonas gingivalis lipopolysaccharide (P.g-LPS), a major pathogen in adult periodontitis. When HL-60-derived neutrophils were stimulated with P.g-LPS, LDs increased in both number and size. In the differentiated cells, PLIN3 was induced while PLIN1, PLIN2 and PLIN5 were not detected. PGE(2) production and the PLIN3 protein level were increased by the P.g-LPS treatment of the cells in a dose-dependent manner. When PLIN3 was down-regulated with siRNA treatment, LDs essentially disappeared and the level of PGE(2) secreted in the cell culture medium decreased by 65%. In addition, the suppression of PLIN3 repressed the PGE(2) producing enzymes; i.e., microsomal PGE synthase-1, -2 and cyclooxygenase-2. These findings indicate that PLIN3 has a pivotal role in LD-biogenesis in HL-60-derived neutrophils, and that PLIN3 is associated with the synthesis and secretion of PGE(2).
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spelling pubmed-37312822013-08-09 Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils Nose, Fuyuki Yamaguchi, Tomohiro Kato, Rina Aiuchi, Toshihiro Obama, Takashi Hara, Shuntaro Yamamoto, Matsuo Itabe, Hiroyuki PLoS One Research Article Cytosolic lipid droplets (LDs), which are now recognized as multifunctional organelles, accumulate in leukocytes under various inflammatory conditions. However, little is known about the characteristic features of LDs in neutrophils. In this study, we show that perilipin-3 (PLIN3; formerly called TIP47) is involved in LD formation and the inflammatory response in HL-60-derived neutrophils. HL-60, a promyelocytic cell line, was differentiated into neutrophils via treatment with all-trans retinoic acid. After differentiation, cells were stimulated with Porphyromonas gingivalis lipopolysaccharide (P.g-LPS), a major pathogen in adult periodontitis. When HL-60-derived neutrophils were stimulated with P.g-LPS, LDs increased in both number and size. In the differentiated cells, PLIN3 was induced while PLIN1, PLIN2 and PLIN5 were not detected. PGE(2) production and the PLIN3 protein level were increased by the P.g-LPS treatment of the cells in a dose-dependent manner. When PLIN3 was down-regulated with siRNA treatment, LDs essentially disappeared and the level of PGE(2) secreted in the cell culture medium decreased by 65%. In addition, the suppression of PLIN3 repressed the PGE(2) producing enzymes; i.e., microsomal PGE synthase-1, -2 and cyclooxygenase-2. These findings indicate that PLIN3 has a pivotal role in LD-biogenesis in HL-60-derived neutrophils, and that PLIN3 is associated with the synthesis and secretion of PGE(2). Public Library of Science 2013-08-01 /pmc/articles/PMC3731282/ /pubmed/23936516 http://dx.doi.org/10.1371/journal.pone.0071542 Text en © 2013 Nose et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nose, Fuyuki
Yamaguchi, Tomohiro
Kato, Rina
Aiuchi, Toshihiro
Obama, Takashi
Hara, Shuntaro
Yamamoto, Matsuo
Itabe, Hiroyuki
Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title_full Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title_fullStr Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title_full_unstemmed Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title_short Crucial Role of Perilipin-3 (TIP47) in Formation of Lipid Droplets and PGE(2) Production in HL-60-Derived Neutrophils
title_sort crucial role of perilipin-3 (tip47) in formation of lipid droplets and pge(2) production in hl-60-derived neutrophils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731282/
https://www.ncbi.nlm.nih.gov/pubmed/23936516
http://dx.doi.org/10.1371/journal.pone.0071542
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