Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent translation initiation. This method entails the mRNA display of trillions of genomic fragments, selection for translation initiation, and high-throughput deep sequencing. We identified >12,000 translat...

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Detalles Bibliográficos
Autores principales: Wellensiek, Brian P., Larsen, Andrew C., Stephens, Bret, Kukurba, Kim, Waern, Karl, Briones, Natalia, Liu, Li, Snyder, Michael, Jacobs, Bertram L., Kumar, Sudhir, Chaput, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731418/
https://www.ncbi.nlm.nih.gov/pubmed/23770754
http://dx.doi.org/10.1038/nmeth.2522
Descripción
Sumario:We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent translation initiation. This method entails the mRNA display of trillions of genomic fragments, selection for translation initiation, and high-throughput deep sequencing. We identified >12,000 translation enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cells.

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