Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent translation initiation. This method entails the mRNA display of trillions of genomic fragments, selection for translation initiation, and high-throughput deep sequencing. We identified >12,000 translat...

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Detalles Bibliográficos
Autores principales: Wellensiek, Brian P., Larsen, Andrew C., Stephens, Bret, Kukurba, Kim, Waern, Karl, Briones, Natalia, Liu, Li, Snyder, Michael, Jacobs, Bertram L., Kumar, Sudhir, Chaput, John C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731418/
https://www.ncbi.nlm.nih.gov/pubmed/23770754
http://dx.doi.org/10.1038/nmeth.2522
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author Wellensiek, Brian P.
Larsen, Andrew C.
Stephens, Bret
Kukurba, Kim
Waern, Karl
Briones, Natalia
Liu, Li
Snyder, Michael
Jacobs, Bertram L.
Kumar, Sudhir
Chaput, John C.
author_facet Wellensiek, Brian P.
Larsen, Andrew C.
Stephens, Bret
Kukurba, Kim
Waern, Karl
Briones, Natalia
Liu, Li
Snyder, Michael
Jacobs, Bertram L.
Kumar, Sudhir
Chaput, John C.
author_sort Wellensiek, Brian P.
collection PubMed
description We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent translation initiation. This method entails the mRNA display of trillions of genomic fragments, selection for translation initiation, and high-throughput deep sequencing. We identified >12,000 translation enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cells.
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spelling pubmed-37314182014-02-01 Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome Wellensiek, Brian P. Larsen, Andrew C. Stephens, Bret Kukurba, Kim Waern, Karl Briones, Natalia Liu, Li Snyder, Michael Jacobs, Bertram L. Kumar, Sudhir Chaput, John C. Nat Methods Article We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent translation initiation. This method entails the mRNA display of trillions of genomic fragments, selection for translation initiation, and high-throughput deep sequencing. We identified >12,000 translation enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cells. 2013-06-16 2013-08 /pmc/articles/PMC3731418/ /pubmed/23770754 http://dx.doi.org/10.1038/nmeth.2522 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wellensiek, Brian P.
Larsen, Andrew C.
Stephens, Bret
Kukurba, Kim
Waern, Karl
Briones, Natalia
Liu, Li
Snyder, Michael
Jacobs, Bertram L.
Kumar, Sudhir
Chaput, John C.
Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title_full Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title_fullStr Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title_full_unstemmed Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title_short Genome-Wide Profiling of Cap-Independent Translation Enhancing Elements in the Human Genome
title_sort genome-wide profiling of cap-independent translation enhancing elements in the human genome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731418/
https://www.ncbi.nlm.nih.gov/pubmed/23770754
http://dx.doi.org/10.1038/nmeth.2522
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