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Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions
ABSTRACT: Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their correspondin...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732077/ https://www.ncbi.nlm.nih.gov/pubmed/23758897 http://dx.doi.org/10.1186/1745-6150-8-12 |
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author | Batagov, Arsen O Kurochkin, Igor V |
author_facet | Batagov, Arsen O Kurochkin, Igor V |
author_sort | Batagov, Arsen O |
collection | PubMed |
description | ABSTRACT: Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3′-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3′-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation activity of mRNAs in recipient cells. REVIEWERS: This article was reviewed by Neil Smalheiser and Sandor Pongor. |
format | Online Article Text |
id | pubmed-3732077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-37320772013-08-03 Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions Batagov, Arsen O Kurochkin, Igor V Biol Direct Discovery Notes ABSTRACT: Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3′-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3′-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation activity of mRNAs in recipient cells. REVIEWERS: This article was reviewed by Neil Smalheiser and Sandor Pongor. BioMed Central 2013-06-07 /pmc/articles/PMC3732077/ /pubmed/23758897 http://dx.doi.org/10.1186/1745-6150-8-12 Text en Copyright © 2013 Batagov and Kurochkin; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Discovery Notes Batagov, Arsen O Kurochkin, Igor V Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title | Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title_full | Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title_fullStr | Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title_full_unstemmed | Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title_short | Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions |
title_sort | exosomes secreted by human cells transport largely mrna fragments that are enriched in the 3′-untranslated regions |
topic | Discovery Notes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732077/ https://www.ncbi.nlm.nih.gov/pubmed/23758897 http://dx.doi.org/10.1186/1745-6150-8-12 |
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