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Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients
Cardiovascular (CV) death remains the largest cause of mortality in dialysis patients, unexplained by traditional risk factors. Endothelial microvesicles (EMVs) are elevated in patients with traditional CV risk factors and acute coronary syndromes while platelet MVs (PMVs) are associated with athero...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732282/ https://www.ncbi.nlm.nih.gov/pubmed/23936542 http://dx.doi.org/10.1371/journal.pone.0072663 |
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author | Burton, James O. Hamali, Hassan A. Singh, Ruchir Abbasian, Nima Parsons, Ruth Patel, Amit K. Goodall, Alison H. Brunskill, Nigel J. |
author_facet | Burton, James O. Hamali, Hassan A. Singh, Ruchir Abbasian, Nima Parsons, Ruth Patel, Amit K. Goodall, Alison H. Brunskill, Nigel J. |
author_sort | Burton, James O. |
collection | PubMed |
description | Cardiovascular (CV) death remains the largest cause of mortality in dialysis patients, unexplained by traditional risk factors. Endothelial microvesicles (EMVs) are elevated in patients with traditional CV risk factors and acute coronary syndromes while platelet MVs (PMVs) are associated with atherosclerotic disease states. This study compared relative concentrations of circulating MVs from endothelial cells and platelets in two groups of dialysis patients and matched controls and investigated their relative thromboembolic risk. MVs were isolated from the blood of 20 haemodialysis (HD), 17 peritoneal dialysis (PD) patients and 20 matched controls. Relative concentrations of EMVs (CD144(+ ve)) and PMVs (CD42b(+ ve)) were measured by Western blotting and total MV concentrations were measured using nanoparticle-tracking analysis. The ability to support thrombin generation was measured by reconstituting the MVs in normal plasma, using the Continuous Automated Thrombogram assay triggered with 1µM tissue factor. The total concentration of MVs as well as the measured sub-types was higher in both patient groups compared to controls (p<0.05). MVs from HD and PD patients were able to generate more thrombin than the controls, with higher peak thrombin, and endogenous thrombin potential levels (p<0.02). However there were no differences in either the relative quantity or activity of MVs between the two patient groups (p>0.3). Dialysis patients have higher levels of circulating procoagulant MVs than healthy controls. This may represent a novel and potentially modifiable mediator or predictor of occlusive cardiovascular events in these patients. |
format | Online Article Text |
id | pubmed-3732282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37322822013-08-09 Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients Burton, James O. Hamali, Hassan A. Singh, Ruchir Abbasian, Nima Parsons, Ruth Patel, Amit K. Goodall, Alison H. Brunskill, Nigel J. PLoS One Research Article Cardiovascular (CV) death remains the largest cause of mortality in dialysis patients, unexplained by traditional risk factors. Endothelial microvesicles (EMVs) are elevated in patients with traditional CV risk factors and acute coronary syndromes while platelet MVs (PMVs) are associated with atherosclerotic disease states. This study compared relative concentrations of circulating MVs from endothelial cells and platelets in two groups of dialysis patients and matched controls and investigated their relative thromboembolic risk. MVs were isolated from the blood of 20 haemodialysis (HD), 17 peritoneal dialysis (PD) patients and 20 matched controls. Relative concentrations of EMVs (CD144(+ ve)) and PMVs (CD42b(+ ve)) were measured by Western blotting and total MV concentrations were measured using nanoparticle-tracking analysis. The ability to support thrombin generation was measured by reconstituting the MVs in normal plasma, using the Continuous Automated Thrombogram assay triggered with 1µM tissue factor. The total concentration of MVs as well as the measured sub-types was higher in both patient groups compared to controls (p<0.05). MVs from HD and PD patients were able to generate more thrombin than the controls, with higher peak thrombin, and endogenous thrombin potential levels (p<0.02). However there were no differences in either the relative quantity or activity of MVs between the two patient groups (p>0.3). Dialysis patients have higher levels of circulating procoagulant MVs than healthy controls. This may represent a novel and potentially modifiable mediator or predictor of occlusive cardiovascular events in these patients. Public Library of Science 2013-08-02 /pmc/articles/PMC3732282/ /pubmed/23936542 http://dx.doi.org/10.1371/journal.pone.0072663 Text en © 2013 Burton et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Burton, James O. Hamali, Hassan A. Singh, Ruchir Abbasian, Nima Parsons, Ruth Patel, Amit K. Goodall, Alison H. Brunskill, Nigel J. Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title | Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title_full | Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title_fullStr | Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title_full_unstemmed | Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title_short | Elevated Levels of Procoagulant Plasma Microvesicles in Dialysis Patients |
title_sort | elevated levels of procoagulant plasma microvesicles in dialysis patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732282/ https://www.ncbi.nlm.nih.gov/pubmed/23936542 http://dx.doi.org/10.1371/journal.pone.0072663 |
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