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The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome

In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the...

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Autores principales: Girton, Jack, Wang, Chao, Johansen, Jørgen, Johansen, Kristen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732332/
https://www.ncbi.nlm.nih.gov/pubmed/23579201
http://dx.doi.org/10.4161/fly.24266
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author Girton, Jack
Wang, Chao
Johansen, Jørgen
Johansen, Kristen M.
author_facet Girton, Jack
Wang, Chao
Johansen, Jørgen
Johansen, Kristen M.
author_sort Girton, Jack
collection PubMed
description In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the total amount of heterochromatic DNA. Heterochromatic DNA levels were manipulated by adding or subtracting a Y chromosome as well as by the difference in the amount of pericentric heterochromatin between the X and Y chromosome. The results showed a direct, linear relationship between the amount of heterochromatic DNA in the genome and the expression of the w marker gene in the 118E-10 pericentric reporter line and that increasing amounts of heterochromatic DNA resulted in increasing amounts of pigment/gene activity. In Drosophila heterochromatic spreading and gene silencing is counteracted by H3S10 phosphorylation by the JIL-1 kinase, and we further demonstrate that the haplo-enhancer effect of JIL-1 is proportional to the amount of total heterochomatin, suggesting that JIL-1's activity is dynamically modulated to achieve a more or less constant balance depending on the levels of heterochromatic factors present.
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spelling pubmed-37323322013-08-06 The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome Girton, Jack Wang, Chao Johansen, Jørgen Johansen, Kristen M. Fly (Austin) Research Paper In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the total amount of heterochromatic DNA. Heterochromatic DNA levels were manipulated by adding or subtracting a Y chromosome as well as by the difference in the amount of pericentric heterochromatin between the X and Y chromosome. The results showed a direct, linear relationship between the amount of heterochromatic DNA in the genome and the expression of the w marker gene in the 118E-10 pericentric reporter line and that increasing amounts of heterochromatic DNA resulted in increasing amounts of pigment/gene activity. In Drosophila heterochromatic spreading and gene silencing is counteracted by H3S10 phosphorylation by the JIL-1 kinase, and we further demonstrate that the haplo-enhancer effect of JIL-1 is proportional to the amount of total heterochomatin, suggesting that JIL-1's activity is dynamically modulated to achieve a more or less constant balance depending on the levels of heterochromatic factors present. Landes Bioscience 2013-04-01 2013-04-01 /pmc/articles/PMC3732332/ /pubmed/23579201 http://dx.doi.org/10.4161/fly.24266 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Girton, Jack
Wang, Chao
Johansen, Jørgen
Johansen, Kristen M.
The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title_full The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title_fullStr The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title_full_unstemmed The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title_short The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
title_sort effect of jil-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732332/
https://www.ncbi.nlm.nih.gov/pubmed/23579201
http://dx.doi.org/10.4161/fly.24266
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