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The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome
In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732332/ https://www.ncbi.nlm.nih.gov/pubmed/23579201 http://dx.doi.org/10.4161/fly.24266 |
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author | Girton, Jack Wang, Chao Johansen, Jørgen Johansen, Kristen M. |
author_facet | Girton, Jack Wang, Chao Johansen, Jørgen Johansen, Kristen M. |
author_sort | Girton, Jack |
collection | PubMed |
description | In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the total amount of heterochromatic DNA. Heterochromatic DNA levels were manipulated by adding or subtracting a Y chromosome as well as by the difference in the amount of pericentric heterochromatin between the X and Y chromosome. The results showed a direct, linear relationship between the amount of heterochromatic DNA in the genome and the expression of the w marker gene in the 118E-10 pericentric reporter line and that increasing amounts of heterochromatic DNA resulted in increasing amounts of pigment/gene activity. In Drosophila heterochromatic spreading and gene silencing is counteracted by H3S10 phosphorylation by the JIL-1 kinase, and we further demonstrate that the haplo-enhancer effect of JIL-1 is proportional to the amount of total heterochomatin, suggesting that JIL-1's activity is dynamically modulated to achieve a more or less constant balance depending on the levels of heterochromatic factors present. |
format | Online Article Text |
id | pubmed-3732332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-37323322013-08-06 The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome Girton, Jack Wang, Chao Johansen, Jørgen Johansen, Kristen M. Fly (Austin) Research Paper In this study we have taken advantage of recent whole genome sequencing studies that have determined the DNA content in the heterochromatic regions of each Drosophila chromosome to directly correlate the effect on position-effect variegation of a pericentric insertion reporter line, 118E-10 with the total amount of heterochromatic DNA. Heterochromatic DNA levels were manipulated by adding or subtracting a Y chromosome as well as by the difference in the amount of pericentric heterochromatin between the X and Y chromosome. The results showed a direct, linear relationship between the amount of heterochromatic DNA in the genome and the expression of the w marker gene in the 118E-10 pericentric reporter line and that increasing amounts of heterochromatic DNA resulted in increasing amounts of pigment/gene activity. In Drosophila heterochromatic spreading and gene silencing is counteracted by H3S10 phosphorylation by the JIL-1 kinase, and we further demonstrate that the haplo-enhancer effect of JIL-1 is proportional to the amount of total heterochomatin, suggesting that JIL-1's activity is dynamically modulated to achieve a more or less constant balance depending on the levels of heterochromatic factors present. Landes Bioscience 2013-04-01 2013-04-01 /pmc/articles/PMC3732332/ /pubmed/23579201 http://dx.doi.org/10.4161/fly.24266 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Research Paper Girton, Jack Wang, Chao Johansen, Jørgen Johansen, Kristen M. The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title | The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title_full | The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title_fullStr | The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title_full_unstemmed | The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title_short | The effect of JIL-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
title_sort | effect of jil-1 on position-effect variegation is proportional to the total amount of heterochromatin in the genome |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732332/ https://www.ncbi.nlm.nih.gov/pubmed/23579201 http://dx.doi.org/10.4161/fly.24266 |
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