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Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease
Von Willebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subty...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732594/ https://www.ncbi.nlm.nih.gov/pubmed/23970904 http://dx.doi.org/10.1155/2013/590329 |
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author | Paro, Marina de Oliveira Ferreira, Cyntia Silva Vieira, Fernanda Silva de Santana, Marcos Aurélio Castro-Borges, William Namen-Lopes, Maria Sueli Silva Leclercq, Sophie Yvette Velloso-Rodrigues, Cibele Guerra de Andrade, Milton Hércules |
author_facet | Paro, Marina de Oliveira Ferreira, Cyntia Silva Vieira, Fernanda Silva de Santana, Marcos Aurélio Castro-Borges, William Namen-Lopes, Maria Sueli Silva Leclercq, Sophie Yvette Velloso-Rodrigues, Cibele Guerra de Andrade, Milton Hércules |
author_sort | Paro, Marina de Oliveira |
collection | PubMed |
description | Von Willebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF. The differential diagnosis of the VWD is a difficult task, relying on a panel of tests aimed to assess the plasma levels and function of the VWF. Here, we propose biochemical approaches for the identification of structural variants of the VWF. A bioinformatic analysis was conducted to design seven peptides among which three were representatives of specific amino acid sequences belonging to normal VWF and four encompassed sequences found in the most common VWD subtype 2B. These peptides were used to immunize mice, after which, peptide-specific immunoglobulins were purified. This resulted in four Ig preparations capable of detecting alterations in the subtype 2B VWD plus additional three antibody fractions targeting the normal VWF. The panel of antibodies could serve many applications among them (1) assessment of VWF: antigen interaction, (2) VWF multimer analysis, and (3) production of monoclonal antibodies against VWF for therapeutic purposes as in thrombotic thrombocytopenic purpura. |
format | Online Article Text |
id | pubmed-3732594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-37325942013-08-22 Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease Paro, Marina de Oliveira Ferreira, Cyntia Silva Vieira, Fernanda Silva de Santana, Marcos Aurélio Castro-Borges, William Namen-Lopes, Maria Sueli Silva Leclercq, Sophie Yvette Velloso-Rodrigues, Cibele Guerra de Andrade, Milton Hércules Int J Pept Research Article Von Willebrand disease (VWD) is an inherited hemorrhagic disorder promoted by either quantitative or qualitative defects of the von Willebrand factor (VWF). The disease represents the most common human coagulopathy afflicting 1.3% of the population. Qualitative defects are subdivided into four subtypes and classified according to the molecular dysfunction of the VWF. The differential diagnosis of the VWD is a difficult task, relying on a panel of tests aimed to assess the plasma levels and function of the VWF. Here, we propose biochemical approaches for the identification of structural variants of the VWF. A bioinformatic analysis was conducted to design seven peptides among which three were representatives of specific amino acid sequences belonging to normal VWF and four encompassed sequences found in the most common VWD subtype 2B. These peptides were used to immunize mice, after which, peptide-specific immunoglobulins were purified. This resulted in four Ig preparations capable of detecting alterations in the subtype 2B VWD plus additional three antibody fractions targeting the normal VWF. The panel of antibodies could serve many applications among them (1) assessment of VWF: antigen interaction, (2) VWF multimer analysis, and (3) production of monoclonal antibodies against VWF for therapeutic purposes as in thrombotic thrombocytopenic purpura. Hindawi Publishing Corporation 2013 2013-07-18 /pmc/articles/PMC3732594/ /pubmed/23970904 http://dx.doi.org/10.1155/2013/590329 Text en Copyright © 2013 Marina de Oliveira Paro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Paro, Marina de Oliveira Ferreira, Cyntia Silva Vieira, Fernanda Silva de Santana, Marcos Aurélio Castro-Borges, William Namen-Lopes, Maria Sueli Silva Leclercq, Sophie Yvette Velloso-Rodrigues, Cibele Guerra de Andrade, Milton Hércules Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title | Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title_full | Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title_fullStr | Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title_full_unstemmed | Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title_short | Antigenic Peptides Capable of Inducing Specific Antibodies for Detection of the Major Alterations Found in Type 2B Von Willebrand Disease |
title_sort | antigenic peptides capable of inducing specific antibodies for detection of the major alterations found in type 2b von willebrand disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732594/ https://www.ncbi.nlm.nih.gov/pubmed/23970904 http://dx.doi.org/10.1155/2013/590329 |
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