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The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR

BACKGROUND: Staphylococcus aureus is a common human pathogen in community- and hospital-acquired infection, and its capsule is involved in pathogenesis. The predominance of 2 capsular polysaccharides types 5 and 8, on the surface of clinical isolates, led to the development of conjugate vaccine (Sta...

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Autores principales: Havaei, Seyed Asghar, Moghim, Sharareh, Bardebari, Ali Mohamadi, Narimani, Tahmineh, Azimian, Amir, Akbari, Mojtaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732887/
https://www.ncbi.nlm.nih.gov/pubmed/23930258
http://dx.doi.org/10.4103/2277-9175.107962
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author Havaei, Seyed Asghar
Moghim, Sharareh
Bardebari, Ali Mohamadi
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
author_facet Havaei, Seyed Asghar
Moghim, Sharareh
Bardebari, Ali Mohamadi
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
author_sort Havaei, Seyed Asghar
collection PubMed
description BACKGROUND: Staphylococcus aureus is a common human pathogen in community- and hospital-acquired infection, and its capsule is involved in pathogenesis. The predominance of 2 capsular polysaccharides types 5 and 8, on the surface of clinical isolates, led to the development of conjugate vaccine (Staph VAX) based on capsular polysacchrides types 5 and 8 conjugated to a carrier protein. The aim of this study was to determine the prevalence of capsular polysaccharides types 5 and 8 Staphylococcus aureus strains among isolates and their comparison with respect to methicillin resistance. MATERIALS AND METHODS: We studied the capsular genotypes of 193 isolates that encompassed both hospital- and community-acquired infection in Al-Zahra Hospital of Isfahan city from 2008 to 2009. Cap5 and 8 genes were detected by PCR method. Methicillin resistance was determined by PCR (mecA) and disk diffusion methods as well. RESULT: In this population (193 cases), most of the clinical isolates (73%) expressed capsular polysaccharide type 5 (24%) and 8 (49%), whereas 27% were non-typeable. The prevalence of MRSA in type 8 was 67.9%, whereas MRSA isolates in the capsular genotype 5 were 22.2%. CONCLUSION: This study Staphylococcus aureus confirms that the prevalence of capsular polysaccharide types (5 and 8) are predominant, and Staphylococcus aureus type 8 is more resistant to methicillin compared to type 5.
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spelling pubmed-37328872013-08-08 The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR Havaei, Seyed Asghar Moghim, Sharareh Bardebari, Ali Mohamadi Narimani, Tahmineh Azimian, Amir Akbari, Mojtaba Adv Biomed Res Original Article BACKGROUND: Staphylococcus aureus is a common human pathogen in community- and hospital-acquired infection, and its capsule is involved in pathogenesis. The predominance of 2 capsular polysaccharides types 5 and 8, on the surface of clinical isolates, led to the development of conjugate vaccine (Staph VAX) based on capsular polysacchrides types 5 and 8 conjugated to a carrier protein. The aim of this study was to determine the prevalence of capsular polysaccharides types 5 and 8 Staphylococcus aureus strains among isolates and their comparison with respect to methicillin resistance. MATERIALS AND METHODS: We studied the capsular genotypes of 193 isolates that encompassed both hospital- and community-acquired infection in Al-Zahra Hospital of Isfahan city from 2008 to 2009. Cap5 and 8 genes were detected by PCR method. Methicillin resistance was determined by PCR (mecA) and disk diffusion methods as well. RESULT: In this population (193 cases), most of the clinical isolates (73%) expressed capsular polysaccharide type 5 (24%) and 8 (49%), whereas 27% were non-typeable. The prevalence of MRSA in type 8 was 67.9%, whereas MRSA isolates in the capsular genotype 5 were 22.2%. CONCLUSION: This study Staphylococcus aureus confirms that the prevalence of capsular polysaccharide types (5 and 8) are predominant, and Staphylococcus aureus type 8 is more resistant to methicillin compared to type 5. Medknow Publications & Media Pvt Ltd 2013-03-06 /pmc/articles/PMC3732887/ /pubmed/23930258 http://dx.doi.org/10.4103/2277-9175.107962 Text en Copyright: © 2013 Havaei http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Original Article
Havaei, Seyed Asghar
Moghim, Sharareh
Bardebari, Ali Mohamadi
Narimani, Tahmineh
Azimian, Amir
Akbari, Mojtaba
The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title_full The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title_fullStr The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title_full_unstemmed The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title_short The comparison of Staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to Al-Zahra Hospital by PCR
title_sort comparison of staphylococcus aureus types 5 and 8 with respect to methicillin resistance in patients admitted to al-zahra hospital by pcr
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3732887/
https://www.ncbi.nlm.nih.gov/pubmed/23930258
http://dx.doi.org/10.4103/2277-9175.107962
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