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Programmed cell death during quinoa perisperm development

At seed maturity, quinoa (Chenopodium quinoa Willd.) perisperm consists of uniform, non-living, thin-walled cells full of starch grains. The objective of the present study was to study quinoa perisperm development and describe the programme of cell death that affects the entire tissue. A number of p...

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Autores principales: López-Fernández, María Paula, Maldonado, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733152/
https://www.ncbi.nlm.nih.gov/pubmed/23833197
http://dx.doi.org/10.1093/jxb/ert170
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author López-Fernández, María Paula
Maldonado, Sara
author_facet López-Fernández, María Paula
Maldonado, Sara
author_sort López-Fernández, María Paula
collection PubMed
description At seed maturity, quinoa (Chenopodium quinoa Willd.) perisperm consists of uniform, non-living, thin-walled cells full of starch grains. The objective of the present study was to study quinoa perisperm development and describe the programme of cell death that affects the entire tissue. A number of parameters typically measured during programmed cell death (PCD), such as cellular morphological changes in nuclei and cytoplasm, endoreduplication, DNA fragmentation, and the participation of nucleases and caspase-like proteases in nucleus dismantling, were evaluated; morphological changes in cytoplasm included subcellular aspects related to starch accumulation. This study proved that, following fertilization, the perisperm of quinoa simultaneously accumulates storage reserves and degenerates, both processes mediated by a programme of developmentally controlled cell death. The novel findings regarding perisperm development provide a starting point for further research in the Amaranthaceae genera, such as comparing seeds with and without perisperm, and specifying phylogeny and evolution within this taxon. Wherever possible and appropriate, differences between quinoa perisperm and grass starchy endosperm—a morphologically and functionally similar, although genetically different tissue—were highlighted and discussed.
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spelling pubmed-37331522013-08-05 Programmed cell death during quinoa perisperm development López-Fernández, María Paula Maldonado, Sara J Exp Bot Research Paper At seed maturity, quinoa (Chenopodium quinoa Willd.) perisperm consists of uniform, non-living, thin-walled cells full of starch grains. The objective of the present study was to study quinoa perisperm development and describe the programme of cell death that affects the entire tissue. A number of parameters typically measured during programmed cell death (PCD), such as cellular morphological changes in nuclei and cytoplasm, endoreduplication, DNA fragmentation, and the participation of nucleases and caspase-like proteases in nucleus dismantling, were evaluated; morphological changes in cytoplasm included subcellular aspects related to starch accumulation. This study proved that, following fertilization, the perisperm of quinoa simultaneously accumulates storage reserves and degenerates, both processes mediated by a programme of developmentally controlled cell death. The novel findings regarding perisperm development provide a starting point for further research in the Amaranthaceae genera, such as comparing seeds with and without perisperm, and specifying phylogeny and evolution within this taxon. Wherever possible and appropriate, differences between quinoa perisperm and grass starchy endosperm—a morphologically and functionally similar, although genetically different tissue—were highlighted and discussed. Oxford University Press 2013-08 2013-07-05 /pmc/articles/PMC3733152/ /pubmed/23833197 http://dx.doi.org/10.1093/jxb/ert170 Text en © The Author [2013]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Paper
López-Fernández, María Paula
Maldonado, Sara
Programmed cell death during quinoa perisperm development
title Programmed cell death during quinoa perisperm development
title_full Programmed cell death during quinoa perisperm development
title_fullStr Programmed cell death during quinoa perisperm development
title_full_unstemmed Programmed cell death during quinoa perisperm development
title_short Programmed cell death during quinoa perisperm development
title_sort programmed cell death during quinoa perisperm development
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733152/
https://www.ncbi.nlm.nih.gov/pubmed/23833197
http://dx.doi.org/10.1093/jxb/ert170
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