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Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro

BACKGROUND: Major-histocompatibility-complex class I-related chain A (MICA) antigens are the ligands of NKG2D, which is an activating or coactivating receptor expressed on human NK cells and CD8(+)T cells. We sought to determine whether MICA expression in human corneal epithelium (HCE) could affect...

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Autores principales: Hong, Jiaxu, Qiu, Ting, Qian, Tingting, Li, Gang, Yu, Xiaobo, Chen, Junyi, Le, Qihua, Sun, Xinghuai, Xu, Jianjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733519/
https://www.ncbi.nlm.nih.gov/pubmed/22475346
http://dx.doi.org/10.1186/1471-2415-12-6
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author Hong, Jiaxu
Qiu, Ting
Qian, Tingting
Li, Gang
Yu, Xiaobo
Chen, Junyi
Le, Qihua
Sun, Xinghuai
Xu, Jianjiang
author_facet Hong, Jiaxu
Qiu, Ting
Qian, Tingting
Li, Gang
Yu, Xiaobo
Chen, Junyi
Le, Qihua
Sun, Xinghuai
Xu, Jianjiang
author_sort Hong, Jiaxu
collection PubMed
description BACKGROUND: Major-histocompatibility-complex class I-related chain A (MICA) antigens are the ligands of NKG2D, which is an activating or coactivating receptor expressed on human NK cells and CD8(+)T cells. We sought to determine whether MICA expression in human corneal epithelium (HCE) could affect the cytotoxicity mediated by NK cells or CD8(+)T cells. METHODS: Cell cultures of HCE were harvested from human donor eyes. Flow cytometric analysis and ELISA was performed to determine the levels of MICA expression on HCE. Then, HCE was transfected with a lentivirus vector expressing MICA and GFP. Flow cytometric analysis, RT-PCR, western blot and ELISA were performed to check the levels of MICA expression. For cytotoxicity testing, allogeneic NK cells and CD8(+)T cells were isolated from peripheral blood mononuclear cells of healthy volunteers by magnetic cell sorting. The cytolytic activity of NK cells and CD8(+)T cells was assessed against MICA-transfected HCE (NK cells: E:T ratio = 3:1; CD8(+)T cells: E:T ratio = 10:1) using the nonradioactive cytotoxicity detection kit lactate deshydrogenase. RESULTS: Surface expression of MICA on corneal epithelium was identified at a low level. A cell line of stable human MICA-transfected corneal epithelium was successfully established. Heightened expression of MICA on HCE was found to promote the cytotoxicity mediated by NK cells or CD8(+)T cells, which could be blocked by an anti-MICA antibody. CONCLUSION: MICA molecules may contribute to cytotoxic responses mediated by activated immune effector cells in corneal epithelium immunity.
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spelling pubmed-37335192013-08-06 Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro Hong, Jiaxu Qiu, Ting Qian, Tingting Li, Gang Yu, Xiaobo Chen, Junyi Le, Qihua Sun, Xinghuai Xu, Jianjiang BMC Ophthalmol Research Article BACKGROUND: Major-histocompatibility-complex class I-related chain A (MICA) antigens are the ligands of NKG2D, which is an activating or coactivating receptor expressed on human NK cells and CD8(+)T cells. We sought to determine whether MICA expression in human corneal epithelium (HCE) could affect the cytotoxicity mediated by NK cells or CD8(+)T cells. METHODS: Cell cultures of HCE were harvested from human donor eyes. Flow cytometric analysis and ELISA was performed to determine the levels of MICA expression on HCE. Then, HCE was transfected with a lentivirus vector expressing MICA and GFP. Flow cytometric analysis, RT-PCR, western blot and ELISA were performed to check the levels of MICA expression. For cytotoxicity testing, allogeneic NK cells and CD8(+)T cells were isolated from peripheral blood mononuclear cells of healthy volunteers by magnetic cell sorting. The cytolytic activity of NK cells and CD8(+)T cells was assessed against MICA-transfected HCE (NK cells: E:T ratio = 3:1; CD8(+)T cells: E:T ratio = 10:1) using the nonradioactive cytotoxicity detection kit lactate deshydrogenase. RESULTS: Surface expression of MICA on corneal epithelium was identified at a low level. A cell line of stable human MICA-transfected corneal epithelium was successfully established. Heightened expression of MICA on HCE was found to promote the cytotoxicity mediated by NK cells or CD8(+)T cells, which could be blocked by an anti-MICA antibody. CONCLUSION: MICA molecules may contribute to cytotoxic responses mediated by activated immune effector cells in corneal epithelium immunity. BioMed Central 2012-04-04 /pmc/articles/PMC3733519/ /pubmed/22475346 http://dx.doi.org/10.1186/1471-2415-12-6 Text en Copyright © 2012 Hong et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hong, Jiaxu
Qiu, Ting
Qian, Tingting
Li, Gang
Yu, Xiaobo
Chen, Junyi
Le, Qihua
Sun, Xinghuai
Xu, Jianjiang
Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title_full Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title_fullStr Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title_full_unstemmed Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title_short Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8(+)T cells to human corneal epithelium in vitro
title_sort heightened expression of mica enhances the cytotoxicity of nk cells or cd8(+)t cells to human corneal epithelium in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733519/
https://www.ncbi.nlm.nih.gov/pubmed/22475346
http://dx.doi.org/10.1186/1471-2415-12-6
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