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Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata

BACKGROUND: Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. METHODO...

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Autores principales: Monteiro, Fernando A., Peretolchina, Tatiana, Lazoski, Cristiano, Harris, Kecia, Dotson, Ellen M., Abad-Franch, Fernando, Tamayo, Elsa, Pennington, Pamela M., Monroy, Carlota, Cordon-Rosales, Celia, Salazar-Schettino, Paz Maria, Gómez-Palacio, Andrés, Grijalva, Mario J., Beard, Charles B., Marcet, Paula L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733668/
https://www.ncbi.nlm.nih.gov/pubmed/23940678
http://dx.doi.org/10.1371/journal.pone.0070974
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author Monteiro, Fernando A.
Peretolchina, Tatiana
Lazoski, Cristiano
Harris, Kecia
Dotson, Ellen M.
Abad-Franch, Fernando
Tamayo, Elsa
Pennington, Pamela M.
Monroy, Carlota
Cordon-Rosales, Celia
Salazar-Schettino, Paz Maria
Gómez-Palacio, Andrés
Grijalva, Mario J.
Beard, Charles B.
Marcet, Paula L.
author_facet Monteiro, Fernando A.
Peretolchina, Tatiana
Lazoski, Cristiano
Harris, Kecia
Dotson, Ellen M.
Abad-Franch, Fernando
Tamayo, Elsa
Pennington, Pamela M.
Monroy, Carlota
Cordon-Rosales, Celia
Salazar-Schettino, Paz Maria
Gómez-Palacio, Andrés
Grijalva, Mario J.
Beard, Charles B.
Marcet, Paula L.
author_sort Monteiro, Fernando A.
collection PubMed
description BACKGROUND: Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. METHODOLOGY AND FINDINGS: A total of 126 cyt b sequences (621 bp long) were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis) were also sequenced for an ND4 fragment (554 bp long) and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4) disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08), monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. CONCLUSIONS: We propose that Groups I–IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species – likely the basal lineage of the T. dimidiata complex, originated ∼8.25 Mya. The evidence presented here strongly supports the proposition that T. dimidiata is a complex of five cryptic species (Groups I–IV plus T. hegneri) that play different roles as vectors of Chagas disease in the region.
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spelling pubmed-37336682013-08-12 Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata Monteiro, Fernando A. Peretolchina, Tatiana Lazoski, Cristiano Harris, Kecia Dotson, Ellen M. Abad-Franch, Fernando Tamayo, Elsa Pennington, Pamela M. Monroy, Carlota Cordon-Rosales, Celia Salazar-Schettino, Paz Maria Gómez-Palacio, Andrés Grijalva, Mario J. Beard, Charles B. Marcet, Paula L. PLoS One Research Article BACKGROUND: Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. METHODOLOGY AND FINDINGS: A total of 126 cyt b sequences (621 bp long) were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis) were also sequenced for an ND4 fragment (554 bp long) and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4) disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08), monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. CONCLUSIONS: We propose that Groups I–IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species – likely the basal lineage of the T. dimidiata complex, originated ∼8.25 Mya. The evidence presented here strongly supports the proposition that T. dimidiata is a complex of five cryptic species (Groups I–IV plus T. hegneri) that play different roles as vectors of Chagas disease in the region. Public Library of Science 2013-08-05 /pmc/articles/PMC3733668/ /pubmed/23940678 http://dx.doi.org/10.1371/journal.pone.0070974 Text en © 2013 Monteiro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Monteiro, Fernando A.
Peretolchina, Tatiana
Lazoski, Cristiano
Harris, Kecia
Dotson, Ellen M.
Abad-Franch, Fernando
Tamayo, Elsa
Pennington, Pamela M.
Monroy, Carlota
Cordon-Rosales, Celia
Salazar-Schettino, Paz Maria
Gómez-Palacio, Andrés
Grijalva, Mario J.
Beard, Charles B.
Marcet, Paula L.
Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title_full Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title_fullStr Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title_full_unstemmed Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title_short Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
title_sort phylogeographic pattern and extensive mitochondrial dna divergence disclose a species complex within the chagas disease vector triatoma dimidiata
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733668/
https://www.ncbi.nlm.nih.gov/pubmed/23940678
http://dx.doi.org/10.1371/journal.pone.0070974
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