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Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response

BACKGROUND: Aphids are among the most destructive pests in temperate climates, causing significant damage on several crops including tomato. We carried out a transcriptomic and proteomic study to get insights into the molecular mechanisms and dynamics of the tomato response to the Macrosyphum euphor...

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Autores principales: Coppola, Valentina, Coppola, Mariangela, Rocco, Mariapina, Digilio, Maria Cristina, D’Ambrosio, Chiara, Renzone, Giovanni, Martinelli, Rosanna, Scaloni, Andrea, Pennacchio, Francesco, Rao, Rosa, Corrado, Giandomenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733717/
https://www.ncbi.nlm.nih.gov/pubmed/23895395
http://dx.doi.org/10.1186/1471-2164-14-515
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author Coppola, Valentina
Coppola, Mariangela
Rocco, Mariapina
Digilio, Maria Cristina
D’Ambrosio, Chiara
Renzone, Giovanni
Martinelli, Rosanna
Scaloni, Andrea
Pennacchio, Francesco
Rao, Rosa
Corrado, Giandomenico
author_facet Coppola, Valentina
Coppola, Mariangela
Rocco, Mariapina
Digilio, Maria Cristina
D’Ambrosio, Chiara
Renzone, Giovanni
Martinelli, Rosanna
Scaloni, Andrea
Pennacchio, Francesco
Rao, Rosa
Corrado, Giandomenico
author_sort Coppola, Valentina
collection PubMed
description BACKGROUND: Aphids are among the most destructive pests in temperate climates, causing significant damage on several crops including tomato. We carried out a transcriptomic and proteomic study to get insights into the molecular mechanisms and dynamics of the tomato response to the Macrosyphum euphorbiae aphid. RESULTS: The time course analysis of aphid infestation indicated a complex, dynamic pattern of gene expression. Several biological functions were affected and genes related to the stress and defence response were the most represented. The Gene Ontology categories of the differentially expressed genes (899) and identified proteins (57) indicated that the tomato response is characterized by an increased oxidative stress accompanied by the production of proteins involved in the detoxification of oxygen radicals. Aphids elicit a defense reaction based on the cross-communication of different hormone-related signaling pathways such as those related to the salicylic acid (SA), jasmonic acid (JA), ethylene and brassinosteroids. Among them, the SA-signaling pathway and stress-responsive SA-dependent genes play a dominant role. Furthermore, tomato response is characterized by a reduced accumulation of photosynthetic proteins and a modification of the expression of various cell wall related genes. CONCLUSIONS: Our work allowed a more comprehensive understanding of the signaling events and the defense dynamics of the tomato response to aphids in a compatible interaction and, based on experimental data, a model of the tomato–aphid molecular interaction was proposed. Considering the rapid advancement of tomato genomics, this information will be important for the development of new protection strategies.
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spelling pubmed-37337172013-08-06 Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response Coppola, Valentina Coppola, Mariangela Rocco, Mariapina Digilio, Maria Cristina D’Ambrosio, Chiara Renzone, Giovanni Martinelli, Rosanna Scaloni, Andrea Pennacchio, Francesco Rao, Rosa Corrado, Giandomenico BMC Genomics Research Article BACKGROUND: Aphids are among the most destructive pests in temperate climates, causing significant damage on several crops including tomato. We carried out a transcriptomic and proteomic study to get insights into the molecular mechanisms and dynamics of the tomato response to the Macrosyphum euphorbiae aphid. RESULTS: The time course analysis of aphid infestation indicated a complex, dynamic pattern of gene expression. Several biological functions were affected and genes related to the stress and defence response were the most represented. The Gene Ontology categories of the differentially expressed genes (899) and identified proteins (57) indicated that the tomato response is characterized by an increased oxidative stress accompanied by the production of proteins involved in the detoxification of oxygen radicals. Aphids elicit a defense reaction based on the cross-communication of different hormone-related signaling pathways such as those related to the salicylic acid (SA), jasmonic acid (JA), ethylene and brassinosteroids. Among them, the SA-signaling pathway and stress-responsive SA-dependent genes play a dominant role. Furthermore, tomato response is characterized by a reduced accumulation of photosynthetic proteins and a modification of the expression of various cell wall related genes. CONCLUSIONS: Our work allowed a more comprehensive understanding of the signaling events and the defense dynamics of the tomato response to aphids in a compatible interaction and, based on experimental data, a model of the tomato–aphid molecular interaction was proposed. Considering the rapid advancement of tomato genomics, this information will be important for the development of new protection strategies. BioMed Central 2013-07-29 /pmc/articles/PMC3733717/ /pubmed/23895395 http://dx.doi.org/10.1186/1471-2164-14-515 Text en Copyright © 2013 Coppola et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Coppola, Valentina
Coppola, Mariangela
Rocco, Mariapina
Digilio, Maria Cristina
D’Ambrosio, Chiara
Renzone, Giovanni
Martinelli, Rosanna
Scaloni, Andrea
Pennacchio, Francesco
Rao, Rosa
Corrado, Giandomenico
Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title_full Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title_fullStr Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title_full_unstemmed Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title_short Transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
title_sort transcriptomic and proteomic analysis of a compatible tomato-aphid interaction reveals a predominant salicylic acid-dependent plant response
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733717/
https://www.ncbi.nlm.nih.gov/pubmed/23895395
http://dx.doi.org/10.1186/1471-2164-14-515
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