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Serum IL-10 as a marker of severe dengue infection

BACKGROUND: Several studies have shown that serum IL-10, IFNγ and MIF are elevated in patients in severe dengue (SD) and could be used as potential biomarkers. We proceeded to determine if these cytokines could be used as biomarkers in a large cohort of adult dengue patients with varying severity of...

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Autores principales: Malavige, Gathsaurie Neelika, Gomes, Laksiri, Alles, Lukmall, Chang, Thashi, Salimi, Maryam, Fernando, Sachie, Nanayakkara, Kushan DL, Jayaratne, SD, Ogg, Graham S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733887/
https://www.ncbi.nlm.nih.gov/pubmed/23883139
http://dx.doi.org/10.1186/1471-2334-13-341
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author Malavige, Gathsaurie Neelika
Gomes, Laksiri
Alles, Lukmall
Chang, Thashi
Salimi, Maryam
Fernando, Sachie
Nanayakkara, Kushan DL
Jayaratne, SD
Ogg, Graham S
author_facet Malavige, Gathsaurie Neelika
Gomes, Laksiri
Alles, Lukmall
Chang, Thashi
Salimi, Maryam
Fernando, Sachie
Nanayakkara, Kushan DL
Jayaratne, SD
Ogg, Graham S
author_sort Malavige, Gathsaurie Neelika
collection PubMed
description BACKGROUND: Several studies have shown that serum IL-10, IFNγ and MIF are elevated in patients in severe dengue (SD) and could be used as potential biomarkers. We proceeded to determine if these cytokines could be used as biomarkers in a large cohort of adult dengue patients with varying severity of dengue infection. METHODS: Serum IL-10 levels were determined in 259 of whom 40 had severe dengue infection. Serum IFNγ and IFNα levels were done in 78 and MIF levels were done in 65 patients with acute dengue infection. Clinical features and laboratory investigations were undertaken during the febrile and critical phase. RESULTS: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD. Serum IL-10 levels significantly and inversely correlated with white cell counts (R = −0.23, p = 0.0002) and lymphocyte counts (R = −0.29, p < 0.0001) but significantly and positively correlated with aspartate tranaminase levels (R = 0.16, p = 0.01) and alanine transaminase levels (R = 0.22, p = 0.007). However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66). Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD. There was no difference (p = 0.34) in serum IFNα levels and serum MIF levels (p = 0.15) in patients with SD and non SD. CONCLUSION: Although serum IL-10 was significantly elevated in patients with SD it had a poor discriminatory value in identifying those with SD and non SD and therefore, is unsuitable to be used as a robust biomarker in this cohort.
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spelling pubmed-37338872013-08-06 Serum IL-10 as a marker of severe dengue infection Malavige, Gathsaurie Neelika Gomes, Laksiri Alles, Lukmall Chang, Thashi Salimi, Maryam Fernando, Sachie Nanayakkara, Kushan DL Jayaratne, SD Ogg, Graham S BMC Infect Dis Research Article BACKGROUND: Several studies have shown that serum IL-10, IFNγ and MIF are elevated in patients in severe dengue (SD) and could be used as potential biomarkers. We proceeded to determine if these cytokines could be used as biomarkers in a large cohort of adult dengue patients with varying severity of dengue infection. METHODS: Serum IL-10 levels were determined in 259 of whom 40 had severe dengue infection. Serum IFNγ and IFNα levels were done in 78 and MIF levels were done in 65 patients with acute dengue infection. Clinical features and laboratory investigations were undertaken during the febrile and critical phase. RESULTS: We found that serum IL-10 levels were significantly higher (p = 0.001) in patients with SD, when compared to those with non SD. Serum IL-10 levels significantly and inversely correlated with white cell counts (R = −0.23, p = 0.0002) and lymphocyte counts (R = −0.29, p < 0.0001) but significantly and positively correlated with aspartate tranaminase levels (R = 0.16, p = 0.01) and alanine transaminase levels (R = 0.22, p = 0.007). However, IL-10 levels did not have a good predictive value in discriminating those who were likely to develop SD (AUC = 0.66). Serum IFNγ levels were also significantly higher (p = 0.04) in patients with SD when compared to non SD. There was no difference (p = 0.34) in serum IFNα levels and serum MIF levels (p = 0.15) in patients with SD and non SD. CONCLUSION: Although serum IL-10 was significantly elevated in patients with SD it had a poor discriminatory value in identifying those with SD and non SD and therefore, is unsuitable to be used as a robust biomarker in this cohort. BioMed Central 2013-07-24 /pmc/articles/PMC3733887/ /pubmed/23883139 http://dx.doi.org/10.1186/1471-2334-13-341 Text en Copyright © 2013 Malavige et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Malavige, Gathsaurie Neelika
Gomes, Laksiri
Alles, Lukmall
Chang, Thashi
Salimi, Maryam
Fernando, Sachie
Nanayakkara, Kushan DL
Jayaratne, SD
Ogg, Graham S
Serum IL-10 as a marker of severe dengue infection
title Serum IL-10 as a marker of severe dengue infection
title_full Serum IL-10 as a marker of severe dengue infection
title_fullStr Serum IL-10 as a marker of severe dengue infection
title_full_unstemmed Serum IL-10 as a marker of severe dengue infection
title_short Serum IL-10 as a marker of severe dengue infection
title_sort serum il-10 as a marker of severe dengue infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733887/
https://www.ncbi.nlm.nih.gov/pubmed/23883139
http://dx.doi.org/10.1186/1471-2334-13-341
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