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Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™

BACKGROUND: Plasmodium knowlesi is a simian malaria parasite that is widespread in humans in Malaysian Borneo. However, little is known about the incidence and distribution of this parasite in the Sandakan division, Malaysian Borneo. Therefore, the aim of the present epidemiological study was to inv...

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Autores principales: Goh, Xiang Ting, Lim, Yvonne AL, Vythilingam, Indra, Chew, Ching Hoong, Lee, Ping Chin, Ngui, Romano, Tan, Tian Chye, Yap, Nan Jiun, Nissapatorn, Veeranoot, Chua, Kek Heng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733977/
https://www.ncbi.nlm.nih.gov/pubmed/23902626
http://dx.doi.org/10.1186/1475-2875-12-264
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author Goh, Xiang Ting
Lim, Yvonne AL
Vythilingam, Indra
Chew, Ching Hoong
Lee, Ping Chin
Ngui, Romano
Tan, Tian Chye
Yap, Nan Jiun
Nissapatorn, Veeranoot
Chua, Kek Heng
author_facet Goh, Xiang Ting
Lim, Yvonne AL
Vythilingam, Indra
Chew, Ching Hoong
Lee, Ping Chin
Ngui, Romano
Tan, Tian Chye
Yap, Nan Jiun
Nissapatorn, Veeranoot
Chua, Kek Heng
author_sort Goh, Xiang Ting
collection PubMed
description BACKGROUND: Plasmodium knowlesi is a simian malaria parasite that is widespread in humans in Malaysian Borneo. However, little is known about the incidence and distribution of this parasite in the Sandakan division, Malaysian Borneo. Therefore, the aim of the present epidemiological study was to investigate the incidence and distribution of P. knowlesi as well as other Plasmodium species in this division based on a most recent developed hexaplex PCR system (PlasmoNex™). METHODS: A total of 189 whole blood samples were collected from Telupid Health Clinic, Sabah, Malaysia, from 2008 to 2011. All patients who participated in the study were microscopically malaria positive before recruitment. Complete demographic details and haematological profiles were obtained from 85 patients (13 females and 72 males). Identification of Plasmodium species was conducted using PlasmoNex™ targeting the 18S ssu rRNA gene. RESULTS: A total of 178 samples were positive for Plasmodium species by using PlasmoNex™. Plasmodium falciparum was identified in 68 samples (38.2%) followed by 64 cases (36.0%) of Plasmodium vivax, 42 (23.6%) cases of P. knowlesi, two (1.1%) cases of Plasmodium malariae and two (1.1%) mixed-species infections (i e, P. vivax/P. falciparum). Thirty-five PlasmoNex™ positive P. knowlesi samples were misdiagnosed as P. malariae by microscopy. Plasmodium knowlesi was detected in all four districts of Sandakan division with the highest incidence in the Kinabatangan district. Thrombocytopaenia and anaemia showed to be the most frequent malaria-associated haematological complications in this study. CONCLUSIONS: The discovery of P. knowlesi in Sandakan division showed that prospective studies on the epidemiological risk factors and transmission dynamics of P. knowlesi in these areas are crucial in order to develop strategies for effective malaria control. The availability of advanced diagnostic tool PlasmoNex™ enhanced the accuracy and accelerated the speed in the diagnosis of malaria.
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spelling pubmed-37339772013-08-06 Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™ Goh, Xiang Ting Lim, Yvonne AL Vythilingam, Indra Chew, Ching Hoong Lee, Ping Chin Ngui, Romano Tan, Tian Chye Yap, Nan Jiun Nissapatorn, Veeranoot Chua, Kek Heng Malar J Research BACKGROUND: Plasmodium knowlesi is a simian malaria parasite that is widespread in humans in Malaysian Borneo. However, little is known about the incidence and distribution of this parasite in the Sandakan division, Malaysian Borneo. Therefore, the aim of the present epidemiological study was to investigate the incidence and distribution of P. knowlesi as well as other Plasmodium species in this division based on a most recent developed hexaplex PCR system (PlasmoNex™). METHODS: A total of 189 whole blood samples were collected from Telupid Health Clinic, Sabah, Malaysia, from 2008 to 2011. All patients who participated in the study were microscopically malaria positive before recruitment. Complete demographic details and haematological profiles were obtained from 85 patients (13 females and 72 males). Identification of Plasmodium species was conducted using PlasmoNex™ targeting the 18S ssu rRNA gene. RESULTS: A total of 178 samples were positive for Plasmodium species by using PlasmoNex™. Plasmodium falciparum was identified in 68 samples (38.2%) followed by 64 cases (36.0%) of Plasmodium vivax, 42 (23.6%) cases of P. knowlesi, two (1.1%) cases of Plasmodium malariae and two (1.1%) mixed-species infections (i e, P. vivax/P. falciparum). Thirty-five PlasmoNex™ positive P. knowlesi samples were misdiagnosed as P. malariae by microscopy. Plasmodium knowlesi was detected in all four districts of Sandakan division with the highest incidence in the Kinabatangan district. Thrombocytopaenia and anaemia showed to be the most frequent malaria-associated haematological complications in this study. CONCLUSIONS: The discovery of P. knowlesi in Sandakan division showed that prospective studies on the epidemiological risk factors and transmission dynamics of P. knowlesi in these areas are crucial in order to develop strategies for effective malaria control. The availability of advanced diagnostic tool PlasmoNex™ enhanced the accuracy and accelerated the speed in the diagnosis of malaria. BioMed Central 2013-07-31 /pmc/articles/PMC3733977/ /pubmed/23902626 http://dx.doi.org/10.1186/1475-2875-12-264 Text en Copyright © 2013 Goh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Goh, Xiang Ting
Lim, Yvonne AL
Vythilingam, Indra
Chew, Ching Hoong
Lee, Ping Chin
Ngui, Romano
Tan, Tian Chye
Yap, Nan Jiun
Nissapatorn, Veeranoot
Chua, Kek Heng
Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title_full Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title_fullStr Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title_full_unstemmed Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title_short Increased detection of Plasmodium knowlesi in Sandakan division, Sabah as revealed by PlasmoNex™
title_sort increased detection of plasmodium knowlesi in sandakan division, sabah as revealed by plasmonex™
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733977/
https://www.ncbi.nlm.nih.gov/pubmed/23902626
http://dx.doi.org/10.1186/1475-2875-12-264
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