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Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia

BACKGROUND: Use of lignocellulosic biomass has received attention lately because it can be converted into various versatile chemical compounds by biological processes. In this study, a two-step pretreatment with dilute sulfuric acid and aqueous ammonia was performed efficiently on rice straw to obta...

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Autores principales: Kim, Sung Bong, Lee, Sang Jun, Lee, Ju Hun, Jung, You Ree, Thapa, Laxmi Prasad, Kim, Jun Seok, Um, Youngsoon, Park, Chulhwan, Kim, Seung Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734028/
https://www.ncbi.nlm.nih.gov/pubmed/23898802
http://dx.doi.org/10.1186/1754-6834-6-109
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author Kim, Sung Bong
Lee, Sang Jun
Lee, Ju Hun
Jung, You Ree
Thapa, Laxmi Prasad
Kim, Jun Seok
Um, Youngsoon
Park, Chulhwan
Kim, Seung Wook
author_facet Kim, Sung Bong
Lee, Sang Jun
Lee, Ju Hun
Jung, You Ree
Thapa, Laxmi Prasad
Kim, Jun Seok
Um, Youngsoon
Park, Chulhwan
Kim, Seung Wook
author_sort Kim, Sung Bong
collection PubMed
description BACKGROUND: Use of lignocellulosic biomass has received attention lately because it can be converted into various versatile chemical compounds by biological processes. In this study, a two-step pretreatment with dilute sulfuric acid and aqueous ammonia was performed efficiently on rice straw to obtain fermentable sugar. The soaking in aqueous ammonia process was also optimized by a statistical method. RESULTS: Response surface methodology was employed. The determination coefficient (R(2)) value was found to be 0.9607 and the coefficient of variance was 6.77. The optimal pretreatment conditions were a temperature of 42.75°C, an aqueous ammonia concentration of 20.93%, and a reaction time of 48 h. The optimal enzyme concentration for saccharification was 30 filter paper units. The crystallinity index was approximately 60.23% and the Fourier transform infrared results showed the distinct peaks of glucan. Ethanol production using Saccharomyces cerevisiae K35 was performed to verify whether the glucose saccharified from rice straw was fermentable. CONCLUSIONS: The combined pretreatment using dilute sulfuric acid and aqueous ammonia on rice straw efficiently yielded fermentable sugar and achieved almost the same crystallinity index as that of α-cellulose.
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spelling pubmed-37340282013-08-06 Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia Kim, Sung Bong Lee, Sang Jun Lee, Ju Hun Jung, You Ree Thapa, Laxmi Prasad Kim, Jun Seok Um, Youngsoon Park, Chulhwan Kim, Seung Wook Biotechnol Biofuels Research BACKGROUND: Use of lignocellulosic biomass has received attention lately because it can be converted into various versatile chemical compounds by biological processes. In this study, a two-step pretreatment with dilute sulfuric acid and aqueous ammonia was performed efficiently on rice straw to obtain fermentable sugar. The soaking in aqueous ammonia process was also optimized by a statistical method. RESULTS: Response surface methodology was employed. The determination coefficient (R(2)) value was found to be 0.9607 and the coefficient of variance was 6.77. The optimal pretreatment conditions were a temperature of 42.75°C, an aqueous ammonia concentration of 20.93%, and a reaction time of 48 h. The optimal enzyme concentration for saccharification was 30 filter paper units. The crystallinity index was approximately 60.23% and the Fourier transform infrared results showed the distinct peaks of glucan. Ethanol production using Saccharomyces cerevisiae K35 was performed to verify whether the glucose saccharified from rice straw was fermentable. CONCLUSIONS: The combined pretreatment using dilute sulfuric acid and aqueous ammonia on rice straw efficiently yielded fermentable sugar and achieved almost the same crystallinity index as that of α-cellulose. BioMed Central 2013-07-30 /pmc/articles/PMC3734028/ /pubmed/23898802 http://dx.doi.org/10.1186/1754-6834-6-109 Text en Copyright © 2013 Kim et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kim, Sung Bong
Lee, Sang Jun
Lee, Ju Hun
Jung, You Ree
Thapa, Laxmi Prasad
Kim, Jun Seok
Um, Youngsoon
Park, Chulhwan
Kim, Seung Wook
Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title_full Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title_fullStr Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title_full_unstemmed Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title_short Pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
title_sort pretreatment of rice straw with combined process using dilute sulfuric acid and aqueous ammonia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734028/
https://www.ncbi.nlm.nih.gov/pubmed/23898802
http://dx.doi.org/10.1186/1754-6834-6-109
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