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Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia

Microtubules are subject to a variety of posttranslational modifications that potentially regulate cytoskeletal functions. Two modifications, glutamylation and glycylation, are highly enriched in the axonemes of most eukaryotes, and might therefore play particularly important roles in cilia and flag...

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Autores principales: Bosch Grau, Montserrat, Gonzalez Curto, Gloria, Rocha, Cecilia, Magiera, Maria M., Marques Sousa, Patricia, Giordano, Tiziana, Spassky, Nathalie, Janke, Carsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734080/
https://www.ncbi.nlm.nih.gov/pubmed/23897886
http://dx.doi.org/10.1083/jcb.201305041
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author Bosch Grau, Montserrat
Gonzalez Curto, Gloria
Rocha, Cecilia
Magiera, Maria M.
Marques Sousa, Patricia
Giordano, Tiziana
Spassky, Nathalie
Janke, Carsten
author_facet Bosch Grau, Montserrat
Gonzalez Curto, Gloria
Rocha, Cecilia
Magiera, Maria M.
Marques Sousa, Patricia
Giordano, Tiziana
Spassky, Nathalie
Janke, Carsten
author_sort Bosch Grau, Montserrat
collection PubMed
description Microtubules are subject to a variety of posttranslational modifications that potentially regulate cytoskeletal functions. Two modifications, glutamylation and glycylation, are highly enriched in the axonemes of most eukaryotes, and might therefore play particularly important roles in cilia and flagella. Here we systematically analyze the dynamics of glutamylation and glycylation in developing mouse ependymal cilia and the expression of the corresponding enzymes in the brain. By systematically screening enzymes of the TTLL family for specific functions in ependymal cilia, we demonstrate that the glycylating enzymes TTLL3 and TTLL8 were required for stability and maintenance of ependymal cilia, whereas the polyglutamylase TTLL6 was necessary for coordinated beating behavior. Our work provides evidence for a functional separation of glutamylating and glycylating enzymes in mammalian ependymal cilia. It further advances the elucidation of the functions of tubulin posttranslational modifications in motile cilia of the mammalian brain and their potential importance in brain development and disease.
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spelling pubmed-37340802014-02-05 Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia Bosch Grau, Montserrat Gonzalez Curto, Gloria Rocha, Cecilia Magiera, Maria M. Marques Sousa, Patricia Giordano, Tiziana Spassky, Nathalie Janke, Carsten J Cell Biol Research Articles Microtubules are subject to a variety of posttranslational modifications that potentially regulate cytoskeletal functions. Two modifications, glutamylation and glycylation, are highly enriched in the axonemes of most eukaryotes, and might therefore play particularly important roles in cilia and flagella. Here we systematically analyze the dynamics of glutamylation and glycylation in developing mouse ependymal cilia and the expression of the corresponding enzymes in the brain. By systematically screening enzymes of the TTLL family for specific functions in ependymal cilia, we demonstrate that the glycylating enzymes TTLL3 and TTLL8 were required for stability and maintenance of ependymal cilia, whereas the polyglutamylase TTLL6 was necessary for coordinated beating behavior. Our work provides evidence for a functional separation of glutamylating and glycylating enzymes in mammalian ependymal cilia. It further advances the elucidation of the functions of tubulin posttranslational modifications in motile cilia of the mammalian brain and their potential importance in brain development and disease. The Rockefeller University Press 2013-08-05 /pmc/articles/PMC3734080/ /pubmed/23897886 http://dx.doi.org/10.1083/jcb.201305041 Text en © 2013 Bosch Grau et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Bosch Grau, Montserrat
Gonzalez Curto, Gloria
Rocha, Cecilia
Magiera, Maria M.
Marques Sousa, Patricia
Giordano, Tiziana
Spassky, Nathalie
Janke, Carsten
Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title_full Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title_fullStr Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title_full_unstemmed Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title_short Tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
title_sort tubulin glycylases and glutamylases have distinct functions in stabilization and motility of ependymal cilia
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734080/
https://www.ncbi.nlm.nih.gov/pubmed/23897886
http://dx.doi.org/10.1083/jcb.201305041
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