Cargando…

Cryo-electron tomography: The challenge of doing structural biology in situ

Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technic...

Descripción completa

Detalles Bibliográficos
Autores principales: Lučić, Vladan, Rigort, Alexander, Baumeister, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734081/
https://www.ncbi.nlm.nih.gov/pubmed/23918936
http://dx.doi.org/10.1083/jcb.201304193
_version_ 1782279468229853184
author Lučić, Vladan
Rigort, Alexander
Baumeister, Wolfgang
author_facet Lučić, Vladan
Rigort, Alexander
Baumeister, Wolfgang
author_sort Lučić, Vladan
collection PubMed
description Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technical advances include sample vitrification that faithfully preserves molecular structures, three-dimensional imaging by electron tomography, and improved image-processing methods. These new techniques have enabled the extraction of high fidelity structural information and are beginning to reveal the macromolecular organization of unperturbed cellular environments.
format Online
Article
Text
id pubmed-3734081
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-37340812014-02-05 Cryo-electron tomography: The challenge of doing structural biology in situ Lučić, Vladan Rigort, Alexander Baumeister, Wolfgang J Cell Biol Reviews Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technical advances include sample vitrification that faithfully preserves molecular structures, three-dimensional imaging by electron tomography, and improved image-processing methods. These new techniques have enabled the extraction of high fidelity structural information and are beginning to reveal the macromolecular organization of unperturbed cellular environments. The Rockefeller University Press 2013-08-05 /pmc/articles/PMC3734081/ /pubmed/23918936 http://dx.doi.org/10.1083/jcb.201304193 Text en © 2013 Lučić et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Reviews
Lučić, Vladan
Rigort, Alexander
Baumeister, Wolfgang
Cryo-electron tomography: The challenge of doing structural biology in situ
title Cryo-electron tomography: The challenge of doing structural biology in situ
title_full Cryo-electron tomography: The challenge of doing structural biology in situ
title_fullStr Cryo-electron tomography: The challenge of doing structural biology in situ
title_full_unstemmed Cryo-electron tomography: The challenge of doing structural biology in situ
title_short Cryo-electron tomography: The challenge of doing structural biology in situ
title_sort cryo-electron tomography: the challenge of doing structural biology in situ
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734081/
https://www.ncbi.nlm.nih.gov/pubmed/23918936
http://dx.doi.org/10.1083/jcb.201304193
work_keys_str_mv AT lucicvladan cryoelectrontomographythechallengeofdoingstructuralbiologyinsitu
AT rigortalexander cryoelectrontomographythechallengeofdoingstructuralbiologyinsitu
AT baumeisterwolfgang cryoelectrontomographythechallengeofdoingstructuralbiologyinsitu