Cargando…
Association analysis between variants in KISS1 gene and litter size in goats
BACKGROUND: Kisspeptins are the peptide products of KISS1 gene, which operate via the G - protein-coupled receptor GPR54. These peptides have emerged as essential upstream regulators of neurons secreting gonadotropin-releasing hormone (GnRH), the major hypothalamic node for the stimulatory control o...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734198/ https://www.ncbi.nlm.nih.gov/pubmed/23915023 http://dx.doi.org/10.1186/1471-2156-14-63 |
_version_ | 1782279494977978368 |
---|---|
author | An, Xiaopeng Ma, Teng Hou, Jinxing Fang, Fang Han, Peng Yan, Yan Zhao, Haibo Song, Yunxuan Wang, Jiangang Cao, Binyun |
author_facet | An, Xiaopeng Ma, Teng Hou, Jinxing Fang, Fang Han, Peng Yan, Yan Zhao, Haibo Song, Yunxuan Wang, Jiangang Cao, Binyun |
author_sort | An, Xiaopeng |
collection | PubMed |
description | BACKGROUND: Kisspeptins are the peptide products of KISS1 gene, which operate via the G - protein-coupled receptor GPR54. These peptides have emerged as essential upstream regulators of neurons secreting gonadotropin-releasing hormone (GnRH), the major hypothalamic node for the stimulatory control of the hypothalamic–pituitary– gonadal (HPG) axis. The present study detected the polymorphisms of caprine KISS1 gene in three goat breeds and investigated the associations between these genetic markers and litter size. RESULTS: Three goat breeds (n = 680) were used to detect single nucleotide polymorphisms (SNPs) in the coding regions with their intron–exon boundaries and the proximal flanking regions of KISS1 gene by DNA sequencing and PCR–RFLP. Eleven novel SNPs (g.384G>A, g.1147T>C, g.1417G>A, g.1428_1429delG, g.2124C>T, g.2270C>T, g.2489T>C, g.2510G>A, g.2540C>T, g.3864_3865delCA and g.3885_3886insACCCC) were identified. It was shown that Xinong Saanen and Guanzhong goat breeds were in Hardy-Weinberg disequilibrium at g.384G>A locus (P < 0.05). Both g.2510G>A and g.2540C>T loci were closely linked in Xinong Saanen (SN), Guanzhong (GZ) and Boer (BG) goat breeds (r(2) > 0.33). The g.384G>A, g.2489T>C, g.2510G>A and g.2540C>T SNPs were associated with litter size (P<0.05). Individuals with AATTAATT combinative genotype of SN breed (SC) and TTAATT combinative genotype of BG breed (BC) had higher litter size than those with other combinative genotypes in average parity. The results extend the spectrum of genetic variation of the caprine KISS1 gene, which might contribute to goat genetic resources and breeding. CONCLUSIONS: This study explored the genetic polymorphism of KISS1 gene, and indicated that four SNPs may play an important role in litter size. Their genetic mechanism of reproduction in goat breeds should be further investigated. The female goats with SC1 (AATTAATT) and BC7 (TTAATT) had higher litter size than those with other combinative genotypes in average parity and could be used for the development of new breeds of prolific goats. Further research on a large number of animals is required to confirm the link with increased prolificacy in goats. |
format | Online Article Text |
id | pubmed-3734198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-37341982013-08-06 Association analysis between variants in KISS1 gene and litter size in goats An, Xiaopeng Ma, Teng Hou, Jinxing Fang, Fang Han, Peng Yan, Yan Zhao, Haibo Song, Yunxuan Wang, Jiangang Cao, Binyun BMC Genet Research Article BACKGROUND: Kisspeptins are the peptide products of KISS1 gene, which operate via the G - protein-coupled receptor GPR54. These peptides have emerged as essential upstream regulators of neurons secreting gonadotropin-releasing hormone (GnRH), the major hypothalamic node for the stimulatory control of the hypothalamic–pituitary– gonadal (HPG) axis. The present study detected the polymorphisms of caprine KISS1 gene in three goat breeds and investigated the associations between these genetic markers and litter size. RESULTS: Three goat breeds (n = 680) were used to detect single nucleotide polymorphisms (SNPs) in the coding regions with their intron–exon boundaries and the proximal flanking regions of KISS1 gene by DNA sequencing and PCR–RFLP. Eleven novel SNPs (g.384G>A, g.1147T>C, g.1417G>A, g.1428_1429delG, g.2124C>T, g.2270C>T, g.2489T>C, g.2510G>A, g.2540C>T, g.3864_3865delCA and g.3885_3886insACCCC) were identified. It was shown that Xinong Saanen and Guanzhong goat breeds were in Hardy-Weinberg disequilibrium at g.384G>A locus (P < 0.05). Both g.2510G>A and g.2540C>T loci were closely linked in Xinong Saanen (SN), Guanzhong (GZ) and Boer (BG) goat breeds (r(2) > 0.33). The g.384G>A, g.2489T>C, g.2510G>A and g.2540C>T SNPs were associated with litter size (P<0.05). Individuals with AATTAATT combinative genotype of SN breed (SC) and TTAATT combinative genotype of BG breed (BC) had higher litter size than those with other combinative genotypes in average parity. The results extend the spectrum of genetic variation of the caprine KISS1 gene, which might contribute to goat genetic resources and breeding. CONCLUSIONS: This study explored the genetic polymorphism of KISS1 gene, and indicated that four SNPs may play an important role in litter size. Their genetic mechanism of reproduction in goat breeds should be further investigated. The female goats with SC1 (AATTAATT) and BC7 (TTAATT) had higher litter size than those with other combinative genotypes in average parity and could be used for the development of new breeds of prolific goats. Further research on a large number of animals is required to confirm the link with increased prolificacy in goats. BioMed Central 2013-08-02 /pmc/articles/PMC3734198/ /pubmed/23915023 http://dx.doi.org/10.1186/1471-2156-14-63 Text en Copyright © 2013 An et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article An, Xiaopeng Ma, Teng Hou, Jinxing Fang, Fang Han, Peng Yan, Yan Zhao, Haibo Song, Yunxuan Wang, Jiangang Cao, Binyun Association analysis between variants in KISS1 gene and litter size in goats |
title | Association analysis between variants in KISS1 gene and litter size in goats |
title_full | Association analysis between variants in KISS1 gene and litter size in goats |
title_fullStr | Association analysis between variants in KISS1 gene and litter size in goats |
title_full_unstemmed | Association analysis between variants in KISS1 gene and litter size in goats |
title_short | Association analysis between variants in KISS1 gene and litter size in goats |
title_sort | association analysis between variants in kiss1 gene and litter size in goats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734198/ https://www.ncbi.nlm.nih.gov/pubmed/23915023 http://dx.doi.org/10.1186/1471-2156-14-63 |
work_keys_str_mv | AT anxiaopeng associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT mateng associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT houjinxing associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT fangfang associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT hanpeng associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT yanyan associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT zhaohaibo associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT songyunxuan associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT wangjiangang associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats AT caobinyun associationanalysisbetweenvariantsinkiss1geneandlittersizeingoats |