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Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants

Genetically encoded voltage-sensitive fluorescent proteins (VSFPs) are being used in neurobiology as non-invasive tools to study synchronous electrical activities in specific groups of nerve cells. Here we discuss our efforts to adapt this “light-based electrophysiology” for use in plant systems. We...

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Autores principales: Matzke, Antonius J. M., Matzke, Marjori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734365/
https://www.ncbi.nlm.nih.gov/pubmed/23964285
http://dx.doi.org/10.3389/fpls.2013.00311
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author Matzke, Antonius J. M.
Matzke, Marjori
author_facet Matzke, Antonius J. M.
Matzke, Marjori
author_sort Matzke, Antonius J. M.
collection PubMed
description Genetically encoded voltage-sensitive fluorescent proteins (VSFPs) are being used in neurobiology as non-invasive tools to study synchronous electrical activities in specific groups of nerve cells. Here we discuss our efforts to adapt this “light-based electrophysiology” for use in plant systems. We describe the production of transgenic plants engineered to express different versions of VSFPs that are targeted to the plasma membrane and internal membranes of root cells. The aim is to optically record concurrent changes in plasma membrane potential in populations of cells and at multiple membrane systems within single cells in response to various stimuli in living plants. Such coordinated electrical changes may globally orchestrate cell behavior to elicit successful reactions of the root as a whole to varying and unpredictable environments. Findings from membrane “potential-omics” can eventually be fused with data sets from other “omics” approaches to forge the integrated and comprehensive understanding that underpins the concept of systems biology.
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spelling pubmed-37343652013-08-20 Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants Matzke, Antonius J. M. Matzke, Marjori Front Plant Sci Plant Science Genetically encoded voltage-sensitive fluorescent proteins (VSFPs) are being used in neurobiology as non-invasive tools to study synchronous electrical activities in specific groups of nerve cells. Here we discuss our efforts to adapt this “light-based electrophysiology” for use in plant systems. We describe the production of transgenic plants engineered to express different versions of VSFPs that are targeted to the plasma membrane and internal membranes of root cells. The aim is to optically record concurrent changes in plasma membrane potential in populations of cells and at multiple membrane systems within single cells in response to various stimuli in living plants. Such coordinated electrical changes may globally orchestrate cell behavior to elicit successful reactions of the root as a whole to varying and unpredictable environments. Findings from membrane “potential-omics” can eventually be fused with data sets from other “omics” approaches to forge the integrated and comprehensive understanding that underpins the concept of systems biology. Frontiers Media S.A. 2013-08-06 /pmc/articles/PMC3734365/ /pubmed/23964285 http://dx.doi.org/10.3389/fpls.2013.00311 Text en Copyright © Matzke and Matzke. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Matzke, Antonius J. M.
Matzke, Marjori
Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title_full Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title_fullStr Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title_full_unstemmed Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title_short Membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
title_sort membrane “potential-omics”: toward voltage imaging at the cell population level in roots of living plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734365/
https://www.ncbi.nlm.nih.gov/pubmed/23964285
http://dx.doi.org/10.3389/fpls.2013.00311
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