Detection of β-Lactamase Residues in Milk by Sandwich ELISA

β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunoso...

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Autores principales: Wang, Wenbing, Liu, Liqiang, Xu, Liguang, Ma, Wei, Kuang, Hua, Xu, Chuanlai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734450/
https://www.ncbi.nlm.nih.gov/pubmed/23812026
http://dx.doi.org/10.3390/ijerph10072688
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author Wang, Wenbing
Liu, Liqiang
Xu, Liguang
Ma, Wei
Kuang, Hua
Xu, Chuanlai
author_facet Wang, Wenbing
Liu, Liqiang
Xu, Liguang
Ma, Wei
Kuang, Hua
Xu, Chuanlai
author_sort Wang, Wenbing
collection PubMed
description β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82–103.13%. The intra- and inter-assay coefficients of variation were 6.21–7.38% and 12.96–13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk.
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spelling pubmed-37344502013-08-06 Detection of β-Lactamase Residues in Milk by Sandwich ELISA Wang, Wenbing Liu, Liqiang Xu, Liguang Ma, Wei Kuang, Hua Xu, Chuanlai Int J Environ Res Public Health Article β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of β-lactamase. The limit of detection and linear dynamic range of the method were 4.17 ng/mL and 5.5–100 ng/mL, respectively. β-Lactamase recovery in pure milk was 96.82–103.13%. The intra- and inter-assay coefficients of variation were 6.21–7.38% and 12.96–13.74%, respectively. Our developed sandwich ELISA can be used as a rapid detection method of β-lactamase in milk. MDPI 2013-06-28 2013-07 /pmc/articles/PMC3734450/ /pubmed/23812026 http://dx.doi.org/10.3390/ijerph10072688 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Wenbing
Liu, Liqiang
Xu, Liguang
Ma, Wei
Kuang, Hua
Xu, Chuanlai
Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title_full Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title_fullStr Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title_full_unstemmed Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title_short Detection of β-Lactamase Residues in Milk by Sandwich ELISA
title_sort detection of β-lactamase residues in milk by sandwich elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734450/
https://www.ncbi.nlm.nih.gov/pubmed/23812026
http://dx.doi.org/10.3390/ijerph10072688
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