Cargando…

Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen

The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1)...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Fengyuan, Shu, Gang, Peng, Xi, Fang, Jing, Chen, Kejie, Cui, Hengmin, Chen, Zhengli, Zuo, Zhicai, Deng, Junliang, Geng, Yi, Lai, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734461/
https://www.ncbi.nlm.nih.gov/pubmed/23839060
http://dx.doi.org/10.3390/ijerph10072834
_version_ 1782279540167409664
author Wang, Fengyuan
Shu, Gang
Peng, Xi
Fang, Jing
Chen, Kejie
Cui, Hengmin
Chen, Zhengli
Zuo, Zhicai
Deng, Junliang
Geng, Yi
Lai, Weimin
author_facet Wang, Fengyuan
Shu, Gang
Peng, Xi
Fang, Jing
Chen, Kejie
Cui, Hengmin
Chen, Zhengli
Zuo, Zhicai
Deng, Junliang
Geng, Yi
Lai, Weimin
author_sort Wang, Fengyuan
collection PubMed
description The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1) group), 0.3 mg/kg AFB(1) + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB(1) + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB(1) + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB(1) significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB(1) increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB(1)-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis.
format Online
Article
Text
id pubmed-3734461
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-37344612013-08-06 Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen Wang, Fengyuan Shu, Gang Peng, Xi Fang, Jing Chen, Kejie Cui, Hengmin Chen, Zhengli Zuo, Zhicai Deng, Junliang Geng, Yi Lai, Weimin Int J Environ Res Public Health Article The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1) group), 0.3 mg/kg AFB(1) + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB(1) + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB(1) + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB(1) significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB(1) increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB(1)-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis. MDPI 2013-07-09 2013-07 /pmc/articles/PMC3734461/ /pubmed/23839060 http://dx.doi.org/10.3390/ijerph10072834 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Fengyuan
Shu, Gang
Peng, Xi
Fang, Jing
Chen, Kejie
Cui, Hengmin
Chen, Zhengli
Zuo, Zhicai
Deng, Junliang
Geng, Yi
Lai, Weimin
Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title_full Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title_fullStr Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title_full_unstemmed Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title_short Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
title_sort protective effects of sodium selenite against aflatoxin b(1)-induced oxidative stress and apoptosis in broiler spleen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734461/
https://www.ncbi.nlm.nih.gov/pubmed/23839060
http://dx.doi.org/10.3390/ijerph10072834
work_keys_str_mv AT wangfengyuan protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT shugang protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT pengxi protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT fangjing protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT chenkejie protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT cuihengmin protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT chenzhengli protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT zuozhicai protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT dengjunliang protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT gengyi protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen
AT laiweimin protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen