Cargando…
Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen
The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1)...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734461/ https://www.ncbi.nlm.nih.gov/pubmed/23839060 http://dx.doi.org/10.3390/ijerph10072834 |
_version_ | 1782279540167409664 |
---|---|
author | Wang, Fengyuan Shu, Gang Peng, Xi Fang, Jing Chen, Kejie Cui, Hengmin Chen, Zhengli Zuo, Zhicai Deng, Junliang Geng, Yi Lai, Weimin |
author_facet | Wang, Fengyuan Shu, Gang Peng, Xi Fang, Jing Chen, Kejie Cui, Hengmin Chen, Zhengli Zuo, Zhicai Deng, Junliang Geng, Yi Lai, Weimin |
author_sort | Wang, Fengyuan |
collection | PubMed |
description | The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1) group), 0.3 mg/kg AFB(1) + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB(1) + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB(1) + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB(1) significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB(1) increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB(1)-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis. |
format | Online Article Text |
id | pubmed-3734461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-37344612013-08-06 Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen Wang, Fengyuan Shu, Gang Peng, Xi Fang, Jing Chen, Kejie Cui, Hengmin Chen, Zhengli Zuo, Zhicai Deng, Junliang Geng, Yi Lai, Weimin Int J Environ Res Public Health Article The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B(1)-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB(1) (AFB(1) group), 0.3 mg/kg AFB(1) + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB(1) + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB(1) + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB(1) significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB(1) increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB(1)-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis. MDPI 2013-07-09 2013-07 /pmc/articles/PMC3734461/ /pubmed/23839060 http://dx.doi.org/10.3390/ijerph10072834 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Wang, Fengyuan Shu, Gang Peng, Xi Fang, Jing Chen, Kejie Cui, Hengmin Chen, Zhengli Zuo, Zhicai Deng, Junliang Geng, Yi Lai, Weimin Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title | Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title_full | Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title_fullStr | Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title_full_unstemmed | Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title_short | Protective Effects of Sodium Selenite against Aflatoxin B(1)-Induced Oxidative Stress and Apoptosis in Broiler Spleen |
title_sort | protective effects of sodium selenite against aflatoxin b(1)-induced oxidative stress and apoptosis in broiler spleen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734461/ https://www.ncbi.nlm.nih.gov/pubmed/23839060 http://dx.doi.org/10.3390/ijerph10072834 |
work_keys_str_mv | AT wangfengyuan protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT shugang protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT pengxi protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT fangjing protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT chenkejie protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT cuihengmin protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT chenzhengli protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT zuozhicai protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT dengjunliang protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT gengyi protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen AT laiweimin protectiveeffectsofsodiumseleniteagainstaflatoxinb1inducedoxidativestressandapoptosisinbroilerspleen |